Effect of in vivo over-expression of KGF by electroporativelytransfected KGF cDNA on the histology of middle ear cholesteatoma.

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In our previous study, we have indicated that keratinocyte growth factor (KGF) plays an important role in middle ear cholesteatoma formation. KGF is a mesenchymal-cell-derived paracrine growth factor that specifically stimulates epithelial cell growth. In this study, we investigated the effects of over-expressed KGF in vivo. As results, a single injection of KGF expression vector coupled with electroporation induced inflammatory reaction and increased proliferative activity of epithelial cells in external auditory canal, and consequently KGF may possibly induce a cholesteatoma formation. Treatment of rats of the long-term model with SU5402, a KGFreceptor inhibitor, prevented cholesteatoma formation.