Cyclosporin A inhibits cell death of cornel endothelial cells by protecting mitochondrial membrane potential

我们证实了H_2O_2诱导培养的人角膜内皮细胞(HCECs)细胞死亡呈剂量依赖性增加。100 nm时，环孢素A(CsA)可使H_2O_2诱导的内皮细胞死亡减少15%，CsA可显著抑制H_2O_2处理的内皮细胞的透性迁移孔(PTP)。100 nM的CsA对过氧化氢处理的小鼠角膜线粒体膜电位有保护作用，环孢素A可保护线粒体膜电位免受氧化应激的影响，减轻角膜内皮细胞的死亡。

We corfirmed H2O2 induced a dose dependent increase in cell death of cultured human corneal endotehelial cells(HCECs). At concentration of 100nM, Cyclosporine A(CsA) attenuated the H2O2-induced cell death of HCECs by 15%.CsA significantly inhibited the permiability trasition pore(PTP) in H2O2 treaed HCECs. The mitochondrial membrane potential of H2O2 treated mouse corneal cup were protected by CsA at concetration of 100nM.Cyclosporine A protected mitochondrial menmbrane potential from oxidative stress, abd attenuated cell death of corneal endothelial cells.