Breakthrough of the pathogenesis and treatment strategy in necrotizing enterocolitis using two-photon laser scanning microscopy based on the alarmin concept.

基于alarmin概念的双光子激光扫描显微镜技术突破坏死性小肠结肠炎的发病机制和治疗策略。

• 批准号: 23792027
• 负责人: KOIKE Yuhki
• 金额: $ 2.16万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792027/
• 关键词: 先天性消化器疾患学; two-photon laser-scanning microscopy (TPLSM); necrotizing enterocolitis (NEC) model; 壊死性腸炎; 2光子レーザー顕微鏡; アラーミン; 敗血症

This research project is making the necrotizing enterocolitis model created in beta-actin-green fluorescent protein transgenic mice observed by two-photon laser-scanning microscopy(TPLSM) , and figuring out the pathogenesis and treatment of this disease using this model and system. As for the preliminary study, we performed the LPS induced septic model mouse, and achieved the observation of dynamic pathology of intestinal microcirculation in this septic model mouse. Nest stage, we tried to performed the necrotizing enterocolitis (NEC) model in neonatal mouse induced by LPS with hypoxia (with or without hypothermia exposure).

该研究项目是在β-肌动蛋白-绿色荧光蛋白转基因小鼠中建立坏死性小肠结肠炎模型，通过双光子激光扫描显微镜（TPLSM）观察，并利用该模型和系统研究该疾病的发病机制和治疗方法。在前期研究中，我们建立了LPS诱导的脓毒症小鼠模型，并对该模型小鼠肠道微循环进行了动态病理学观察。本研究拟通过LPS诱导新生小鼠坏死性小肠结肠炎（NEC）模型，并结合低氧（低温暴露和非低温暴露）进行实验研究。

项目成果

2光子レーザー顕微鏡を用いた敗血症モデルマウスにおけるNeutrophil extracellular trapsの観察と検討

双光子激光显微镜对脓毒症模型小鼠中性粒细胞胞外陷阱的观察和研究

• 发表时间: 2013
• 作者: Koike Y;Tanaka K;Okugawa Y;Morimoto Y;Toiyama Y;Uchida K;Miki C;Mizoguchi A;Kusunoki M;小池勇樹;小池 勇樹
• 通讯作者: 小池 勇樹

In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy.

• DOI: 10.1155/2012/265487
• 发表时间: 2012
• 期刊: Journal of oncology
• 作者: Tanaka K;Morimoto Y;Toiyama Y;Matsushita K;Kawamura M;Koike Y;Okugawa Y;Inoue Y;Uchida K;Araki T;Mizoguchi A;Kusunoki M
• 通讯作者: Kusunoki M

二光子レーザー顕微鏡を用いた敗血症モデルマウスにおける Neutrophil extracellular trapsの観察と検討

双光子激光显微镜对脓毒症模型小鼠中性粒细胞胞外陷阱的观察和研究

• 发表时间: 2013
• 作者: Koike Y;Tanaka K;Okugawa Y;Morimoto Y;Toiyama Y;Uchida K;Miki C;Mizoguchi A;Kusunoki M;小池勇樹
• 通讯作者: 小池勇樹

In vivo real-time two-photon microscopic imaging of platelet aggregation induced by selective laser irradiation to the endothelium created in the beta-actin-green fluorescent protein transgenic mice

• DOI: 10.1007/s11239-011-0600-y
• 发表时间: 2011-08-01
• 期刊: JOURNAL OF THROMBOSIS AND THROMBOLYSIS
• 影响因子: 4
• 作者: Koike, Yuhki;Tanaka, Koji;Kusunoki, Masato
• 通讯作者: Kusunoki, Masato

二光子レーザー顕微鏡を用いたマウス敗血症モデルの消化管微小循環における病態進展メカニズムの検討

使用双光子激光显微镜检查小鼠脓毒症模型胃肠道微循环疾病进展的机制

• 发表时间: 2012
• 作者: Koike Y;Tanaka K;Okugawa Y;Morimoto Y;Toiyama Y;Uchida K;Miki C;Mizoguchi A;Kusunoki M;小池勇樹;小池 勇樹;小池勇樹
• 通讯作者: 小池勇樹