Basic study of soft tissue augmentation by adipose-inductive biomaterial

重建手术包括自体组织移植的体积增大。然而，作为供体部位的健康区域被破坏，自体组织像拼凑物一样被转移。我们已经尝试在人工生物材料中诱导脂肪生成活性，这种材料可以用注射针注射用于组织增强。首先，用脂肪前体细胞检测最佳剂量下PPAR-g mRNA在体外的表达水平。然后调整加入吡格列酮的三文鱼胶原蛋白的剂量，在体内用注射针将三文鱼胶原蛋白注射到小鼠背部。植入后4周，用成熟脂肪细胞代替吡格列酮胶原凝胶，与不含吡格列酮的对照胶原凝胶进行比较。这些结果表明，使用吡格列酮作为脂肪诱导物质的胶原蛋白促进脂肪形成的可能性。

Reconstructive surgery involves volume augmentation with autologous tissue transfer. However, a healthy region is damaged as a donor site, and the autologous tissue is transferred like a patchwork. We have attempted to induce adipogenesis activity in artificial biomaterial that is injectable with an injection needle for tissue augmentation. First of all, the optimal dose of pioglitazone hydrochloride was examined with adipo-precursor cells in terms of the PPAR-g mRNA expression levels affected by reagent in vitro. Then, salmon collagen with pioglitazone was adjusted in terms of the dose and the salmon collagen was injected into mouse back using an injection needle in vivo. At 4 weeks after implantation, the pioglitazone collagen gel was substituted by mature adipocytes in comparison with the case for control collagen gel without pioglitazone. These results are indicative of the possibility of promoting adipogenesis using collagen with pioglitazone as an adipose-inductive substance.