A basic study of de novo function of PTH in mineral metabolism disorders

PTH在矿物质代谢紊乱中的从头功能基础研究

• 批准号: 23792105
• 负责人: MINAMIZAKI Tomoko
• 金额: $ 2.66万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792105/
• 关键词: 細胞・組織; 骨代謝; リン代謝; PTH; FGF23; 骨芽細胞

PTH increased the promoter activity of Fgf23 in rat calvaria-derived osteoblast cell cultures, however, there was no significant change in the levels of Fgf23mRNA expression and production. In combination with dihydroxyvitamin D3(1,25D), on the other hand, PTH decreased 1,25D-induced Fgf23mRNA expression and production. One of the reasons for this effect is that the expression of vitamin D receptor and type III sodium-dependent phosphate transporter (Pit1) was suppressed by PTH. Further, PTH promoted MKP1 phosphorylation via PKA pathway, and thereby attenuated the signal FGF23 signaling.

PTH可增强大鼠颅骨成骨细胞FGF23的启动子活性，但对Fgf23的表达和产量无明显影响。与1，25D联合应用时，PTH可降低1，25D诱导的Fgf23mRNA的表达和产量。这种作用的原因之一是PTH抑制维生素D受体和III型钠依赖磷酸转运体(PIT1)的表达。此外，PTH通过PKA途径促进Mkp1的磷酸化，从而减弱FGF23信号转导。

项目成果

Transient but not Constitutive Activation of ERK Is Necessary for the Unique Action of FGF23 in Bone.

ERK 的短暂而非持续激活对于 FGF23 在骨中的独特作用是必要的。

• 发表时间: 2012
• 作者: Tomoko Minamizaki;Yukiko Konishi;Hirotaka Yoshioka;Kozai Kozai;Yuji Yoshiko
• 通讯作者: Yuji Yoshiko

Phosphate Regulates Fibromodulin Expression Through the ERK Pathway in Ameloblasts

磷酸盐通过 ERK 途径调节成釉细胞中纤维调节蛋白的表达

• 发表时间: 2012
• 作者: Yoshioka Hirotaka;Yoshiko Yuji;Irie Yasumasa;Minamizaki Tomoko;Kato Yukio;Sugiyama Toshihiro;Maeda Norihiko
• 通讯作者: Maeda Norihiko

Transient but not Constitutive Activation of ERK Is Necessary for the Unique Action of FGF23 in Bone

ERK 的短暂而非组成性激活对于 FGF23 在骨中的独特作用是必要的

• 发表时间: 2012
• 作者: Minamizaki Tomoko;Konishi Yukiko;Yoshioka Hirotaka;Kozai Katsuyuki;Yoshiko Yuji
• 通讯作者: Yoshiko Yuji

MEPE=derived phosphorylated ASARM is involved in FGF23/soluble Klotho-dependent hypomineralization

MEPE=衍生的磷酸化 ASARM 参与 FGF23/可溶性 Klotho 依赖性低矿化

• 发表时间: 2011
• 作者: Konishi Yukiko;Yoshiko Yuji;MInamizaki Tomoko;Yoshioka Hirotaka;Kozai Katsuyuki;Aubin Jane E;Maeda Norihiko
• 通讯作者: Maeda Norihiko

Incisor enamel formation is impaired in transgenic rats overexpressing the type III NaPi transporter Slc20a1.

过度表达 III 型 NaPi 转运蛋白 Slc20a1 的转基因大鼠的门牙釉质形成受到损害。

• 发表时间: 2011
• 期刊: Calcified Tissue International
• 影响因子: 4.2
• 作者: Yoshioka H;Yoshiko Y;Minamizaki T;Suzuki S;Koma Y;Nobukiyo A;Sotomaru Y;Suzuki A;Itho M;Maeda N.
• 通讯作者: Maeda N.