Physiological roles ofpost-translational modifications of water channel aquaporin-5 in the salivary glands.

唾液腺中水通道 aquaporin-5 翻译后修饰的生理作用。

• 批准号: 23792124
• 负责人: HASEGAWA Takahiro
• 金额: $ 2.58万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792124/
• 关键词: 口腔生理学; アクアポリン; 翻訳後修飾; ユビキチン化; リン酸化; 唾液腺; 細胞内膜輸送

Two types of post-translational modifications, phosphorylation and short-chain ubiquitination, of water channel aquaporin-5 (AQP5) were investigated in the salivary gland cells. We reported that AQP5 is rapidly and transiently phosphorylated at the plasma membrane through the cAMP signaling pathway and this phosphorylation may not be involved in the intracellular AQP5 trafficking. A role of Ca2+-induced short-chain ubiquitination of AQP5 could not be cleared because of technical difficulties, small amount of its ubiquitination and the constitutive internalization of AQP5. However, we found that phosphorylation and ubiquitination of AQP5 affect each other’s modification.

在唾液腺细胞中研究了水通道水通道蛋白-5 (AQP5) 的两种类型的翻译后修饰：磷酸化和短链泛素化。我们报道AQP5通过cAMP信号通路在质膜上快速、短暂地磷酸化，并且这种磷酸化可能不参与细胞内AQP5运输。由于技术困难、其泛素化量小以及AQP5的组成型内化，Ca2+诱导的AQP5短链泛素化的作用无法被清除。然而，我们发现AQP5的磷酸化和泛素化会互相影响彼此的修饰。

项目成果

唾液腺細胞におけるアクアポリン 5 の翻訳後修

唾液腺细胞中水通道蛋白5的翻译后修饰

• 发表时间: 2011
• 作者: Toyoda;H.;Saito;M.;Sato;H.;Tanaka;T.;Ogawa;T.;Yatani;H.;Kawano;T.;Kanematsu;T.;Hirata;M. & Kang;Y.;長谷川敬展
• 通讯作者: 長谷川敬展