Regulation of posttranslational modification of water channel aquaporin-5 in the intracellular localization and trafficking

水通道aquaporin-5在细胞内定位和运输中翻译后修饰的调控

• 批准号: 21791806
• 负责人: HASEGAWA Takahiro
• 金额: $ 2.5万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791806/
• 关键词: アクアポリン; 翻訳後修飾; リン酸化; ユビキチン化; 細胞内膜輸送; 唾液腺; アクアポリン5; アクアポリン5(AQP5); 細胞内輸送

Two types of posttranslational modification (phosphorylation and ubiquitination) of water channel aquqaporin-5 (AQP5) were found in mouse salivary gland cells. AQP5 was transiently ubiquitinated and phosphorylated through the stimulation of intracellular Ca^<2+> signaling and the cAMP signaling, respectively. Our present study reveals that the posttranslational modifications of AQP5 are involved in the saliva secretion that is regulated by sympathetic and parasympathetic nervous systems.

水通道蛋白5（AQP5）在小鼠唾液腺细胞中存在两种翻译后修饰（磷酸化和泛素化）。AQP5分别通过胞内Ca^2+信号和cAMP信号的刺激而瞬时泛素化和磷酸化。我们的研究表明，AQP5的翻译后修饰参与唾液分泌，这是由交感神经和副交感神经系统的调节。

项目成果

Effects of naturally occurring G103D point mutation of AQP5 on its water permeability, trafficking and cellular localization in the submandibular gland of rats

• DOI: 10.1042/bc20100086
• 发表时间: 2011-02
• 期刊: Biology of the Cell
• 影响因子: 2.7
• 作者: M. Karabasil;T. Hasegawa;A. Azlina;N. Purwanti;C. Yao;T. Akamatsu;S. Tomioka;K. Hosoi

ホームページ等。

主页等

アクアポリン5のユビキチン化とその細胞内輸送系における役割

水通道蛋白5的泛素化及其在细胞内转运系统中的作用

• 发表时间: 2009
• 作者: 長谷川敬展;アズリナアハマド;ジャフランフルジャフ;廣島佑香;姚陳娟;赤松徹也;細井和雄;Trianna Wahyu Utami;長谷川敬展
• 通讯作者: 長谷川敬展

Roles of lysosomal proteolytic systems in AQP5 degradation in the submandibular gland of rats following chorda tympani parasympathetic denervation

• DOI: 10.1152/ajpgi.00194.2010
• 发表时间: 2010-11-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
• 影响因子: 4.5
• 作者: Azlina, Ahmad;Javkhlan, Purevjav;Hosoi, Kazuo
• 通讯作者: Hosoi, Kazuo

Effects of natural point mutation of rat aquaporin 5 expressed in vitro on its capacity of water permeability and membrane trafficking.

体外表达的大鼠水通道蛋白5天然点突变对其透水性和膜运输能力的影响。

• 发表时间: 2009
• 期刊: The Journal of Medical Investigation 56 Suppl.
• 作者: Karabasil;et al.
• 通讯作者: et al.