Study of inflammatory regulation by biotin via holocarboxylase synthetase

生物素是一种水溶性B族复合维生素，存在于所有生物体中。为了阐明生物素调节炎症的机制，我们构建了一个全新羧化酶合成酶敲除巨噬细胞（HCS-KD细胞）。全新羧化酶合成酶（HCS）是细胞生物素代谢的关键酶。HCS-KD细胞中肿瘤坏死因子-α（一种与炎症有关的重要生物活性蛋白）的产生减少。另一方面，与炎症有关的黏附蛋白CD11b在HCS-KD细胞中表达上调。

Biotin is a water-soluble B complex vitamin found in all organisms. To elucidate the mechanisms of inflammatory regulation by biotin, a holocarboxylase synthetase knock-down macrophage (HCS-KD cells) was constructed. The holocarboxylase synthetase (HCS) is a key-enzyme in cellular biotin metabolisms. The production of tumor necrosis factor-α, an important bioactive protein that involved with inflammation, was decreased in the HCS-KD cells. On the other hand, CD11b, an adhesive protein that involved with inflammation, was up-regulated in the HCS-KD cells.