Development of a new root canal irrigation system using nanobubbles and its clinical application

新型纳米气泡根管冲洗系统的研制及其临床应用

• 批准号: 23792163
• 负责人: HENMI Kouichi
• 金额: $ 1.83万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792163/
• 关键词: ナノバブル; 根管洗浄; Enterococcus fecalis; 次亜塩素酸ナトリウム溶液; 超音波; Enterococcus Faecalis; 透過型電子顕微鏡; 低濃度次亜塩素酸ナトリウム溶液; 殺菌

The objectives of this study were development of a new root canal irrigation system using nanobubbles and its clinical application. A simple and reliable protocol to detect the number of bacteria measuring their ATP activity which was converted to luciferase activity was established. Next, concentration of sodium hypochlorite which is not harmful to tissues was determined to be 0.5%. Enhanced effects of nanobubbles conjunction with ultrasounds were determined. The bacterial solution was placed in the disposable pipet tips of 200ul which were used as artificial root canals. Sodium hypochlorite (0.5%) in the presence or absence of nanobubbles with ultrasounds was applied to the bacterial solution. Nanobubbles with ultrasounds significantly enhanced the bactericidal effects of low concentrated sodium hypochlorite. Morphological effects of nanobubbles with ultrasounds for bacteria were evaluated by transmission electron microscopy (TEM). There was no morphological change on the surface of Enterococcus faecalis (EF) by ultrasound or nanobubbles only, however the destruction of the surface of EF was induced by nanobubbles with ultrasound. Destruction of the cell surface of bacteria by nanobubbles with ultrasound may be involved in the enhancement of bactericidal effects of low concentrated sodium hypochlorite.

本研究的目的是开发一种使用纳米气泡的新型根管冲洗系统及其临床应用。建立了一个简单可靠的方案来检测细菌数量，测量其 ATP 活性，并将其转换为荧光素酶活性。接下来，确定对组织无害的次氯酸钠的浓度为0.5％。确定了纳米气泡与超声波结合的增强效果。将菌液置于200ul的一次性移液器吸头中，用作人工根管。在存在或不存在纳米气泡的情况下，通过超声波将次氯酸钠（0.5%）施加到细菌溶液中。超声波纳米气泡显着增强了低浓度次氯酸钠的杀菌效果。通过透射电子显微镜（TEM）评估超声纳米气泡对细菌的形态影响。超声或纳米气泡单独处理时，粪肠球菌（EF）表面没有形态变化，但纳米气泡联合超声诱导了EF表面的破坏。超声波纳米气泡对细菌细胞表面的破坏可能与低浓度次氯酸钠杀菌效果的增强有关。

项目成果

低濃度次亜塩素酸ナトリウム溶液にナノ バブルを併用した新しい根管洗浄法の開発

纳米气泡结合低浓度次氯酸钠溶液开发新的根管清洁方法

• 发表时间: 2011
• 作者: 辺見浩一;川島伸之;鈴木考尚;山下直 也;立花克郎;中島美砂子;須田英明
• 通讯作者: 須田英明

ナノバブルを作用させたEnterococcus feacalisの透過型電子顕微鏡を用いた観察

使用透射电子显微镜观察纳米气泡处理的粪肠球菌

• 发表时间: 2012
• 作者: 辺見浩一;川島伸之;市野瀬志津子;立花克朗;中島美砂子;須田英明
• 通讯作者: 須田英明

ナノバブルを作用させたEnterococcus faecalisの透過型電子顕微鏡を用いた観察

使用透射电子显微镜观察纳米气泡处理后的粪肠球菌

• 发表时间: 2012
• 作者: 辺見浩一;川島伸之;市野瀬志津子;立花克朗;中島美砂子;須田英明;辺見浩一
• 通讯作者: 辺見浩一

Effects of nano-bubbles on Enterococcus faecalis: transmission electron microscopic investigation

纳米气泡对粪肠球菌的影响：透射电子显微镜研究

• 发表时间: 2013
• 作者: 辺見浩一;川島伸之;中島美砂子;須田英明;辺見浩一
• 通讯作者: 辺見浩一

ナノバブルを併用した新しい根管洗浄法に関する基礎的研究

纳米气泡新型根管清洁方法的基础研究

• 发表时间: 2011
• 作者: 辺見浩一;川島伸之;須田英明;鈴木考尚;山下直也;立花克朗;中島美砂子
• 通讯作者: 中島美砂子