Elucidation of dental pulp cell differentiation by laser

牙髓炎症与龋病的发展有关，可促进牙髓组织产生炎性细胞因子，如白细胞介素-1b （IL-1b）和酶，如基质金属蛋白酶（MMPs），导致组织破坏。本实验结果表明，MEK1/2和ERK1/2通路参与了IL-1b刺激下HPFs中MMP-3的产生。这些样品包括25个实验组样品，辐照时间分别为15、30秒、1、3、5分钟，辐照输出为0.5、1、2、5、10 w，以及1个未辐照的对照组样品。然而，对于0.5 - 10-W高能半导体激光器，在照射3分钟后观察到细胞数量由于细胞死亡而减少，并且在10-W激光照射5分钟后没有发现细胞播种后立即粘附在板表面。

Dental pulp inflammation associated with the progression of dental caries facilitates the production of inflammatory cytokines, such as interleukin-1b (IL-1b), and enzymes, such as matrix metalloproteinases (MMPs), in the pulp tissue, resulting in tissue destruction. The results of this experiment suggest that MEK1/2 and ERK1/2 pathways were involved in the MMP-3 production enhanced by IL-1b stimulation in HPFs.These samples consisted of 25 experimental group samples with 15 and 30-second, and 1, 3, and 5-minute irradiation times, and 0.5, 1, 2, 5, and 10-W irradiation outputs, and one control group sample without irradiation.However, for 0.5 - 10-W high-energy Semiconductor lasers, a decrease in the cell number due to cell death was observed after 3-minute irradiation, and no cell adhesion to the plate surface immediately after cell seeding was noted after 5-minute irradiation with the 10-W laser.