Preparation and evaluation of drug-releasing collagen substrates for oral guided tissue regeneration.

以1型胶原为原料，以GA和EDC为交联剂，制备了具有药物释放功能的交联海绵状胶原。通过改变交联剂的浓度和反应时间，制备出不同交联度的海绵状胶原蛋白。交联速率随交联剂浓度和反应时间的增加而增加，但并不反映在SEM观察到的形貌上。由于海藻酸钠对器官的损伤较大，因此EDC可以作为交联剂。观察人牙周膜成纤维细胞（HPdLF）在藻酸盐凝胶上的增殖情况。以亚甲基蓝为模型药物，通过光学监测法考察了该复合材料的释药性能。这些结果表明海藻酸钠凝胶具有细胞增殖和药物释放的能力。

Aiming to develop a bioabsorbing scaffold material from type 1 collagen, crosslinked spongy collagen was prepared using either GA or EDC as crosslinker, to which drug-releasing function was expected. Both the concentration of crosslinker and the reaction time ware varied to prepare the spongy collagen with different degree of crosslinking. While the crosslinking rate increased with the crosslinker concentration and the reaction time, it was not reflected to the appearance observed by SEM. Since GA is unfavorable to the organ, EDC may be a good alternative as the crosslinker.Alginate gel was obtained by crosslinking sodium alginate using CaCl2. Multiplication of human periodontal ligament fibroblasts (HPdLF) was observed on the prepared alginate gel. Drug-releasing function was investigated by optically monitoring the release of methylene blue as a model drug. These results suggested that alginate gel has an ability of multiplication of cell and drug delivery.