Development of specific molecular target therapy for intractable disease in the oral and maxillofacial Region using the nanobubble and ultrasound

聚乙二醇化(PEG)脂质体避免了网状内皮系统的摄取，从而改善了对肿瘤的药物输送，同时降低了全身毒性。含有阿霉素的聚乙二醇脂质体Doxil已在临床上用于治疗卡波西肉瘤和卵巢癌。Doxil(R)以稳定的状态在体内循环，并通过被动靶向在肿瘤中蓄积。尽管阿霉素在肿瘤内蓄积后很难释放，但我们从Doxi(R)中制备了新的泡沫脂质体，并研究了这种新的Doxil(R)泡沫脂质体、包裹的阿霉素泡沫脂质体(EDBL)和超声波对人牙龈鳞状细胞癌Ca9-22细胞的细胞毒性作用。EDBL与超声联合应用可增强阿霉素对Ca9-22细胞的杀伤作用。这一发现表明，EDBL和超声联合应用可能是一种有效的抗肿瘤药物传递系统。

Polyethylene glycosylated (PEG) liposome avoids uptake by the reticulo-endothelial system, thus improving drug delivery to tumor while decreasing systemic toxicity. PEGliposomes containing doxorubicin, Doxil, have been used to treat Kaposi's sarcoma and ovarian cancer in clinics. Doxil(R) circulates around the body at a stable state and accumulates in tumor by passive targeting. Although it is difficult to release doxorubicin after accumulation into the tumor, we made new bubble liposomes from Doxi(R) and investigated the effect on cytotoxicity in human gingival squamous cell carcinoma Ca9-22 cells by combination with this new Doxil(R) bubble liposome,encapsulated doxorubicin bubble liposomes (EDBL), and ultrasound. The combination of EDBL and ultrasound was found to enhance the cytotoxity of Ca9-22 cells treated by doxorubicin. This findings suggest that combination of EDBL and ultrasound might be an effective drug delivery system against cancer cells.