Development of the oral cancer diagnosis system model by the tumor angiography using the nanobubble and ultrasound

我们开发了一种基于纳米气泡的肿瘤深度诊断系统，该系统可用于口腔癌的诊断、治疗。制作4-硝基喹啉- 1-氧化物致舌癌大鼠模型。在超声监测下，引入带金属芯的弹性针，在距最深肿瘤侵袭前沿5mm处标记手术深缘。手术切除采用弹性针作为最深的手术清除标记。将切除的标本立即浸入明胶溶液中，保持其原有的形状和方向，并冷藏固化。超声观察明胶包埋标本，以确认手术清除。术中可以很容易地证实切除标本的立即手术清除。此外，超声标记显示与组织学边缘有良好的相关性。

We develop the system which can diagnose a tumor depth using the new nanobubble which is applicable for a diagnosis, treatment of oral cancer. We made 4-Nitroquinoline 1-Oxide-induced tongue cancer rat model. Under ultrasonographic monitoring, elastic needles with a metal core were introduced to mark a deep surgical margin of 5 mm from the deepest tumor invasion front. Surgical resection was carried out using elastic needle as the deepest surgical clearance mark. The resected specimen was immediately immersed in gelatin solution maintaining its original shape and orientation, and refrigerated to solidify. Ultrasonographic observations of the gelatin embedded specimens were performed to confirm the surgical clearance. Immediate surgical clearance of the resected specimens could be easily verified intraoperatively. Further, sonographic marking showed a good correlation with the histological margins.