New strategy for bone regeneration based on RNA silencing of GPCR-B

本项目的目的是探索GPCR-B信号转导在骨疾病的新治疗方法中的可能实施，使用体外方法。肠促胰岛素受体GLP-1受体（GLP-1 R）和GIP受体（GIPR）属于GPCR-B家族，在骨代谢中发挥重要作用。发现成骨细胞系MC 3 T3-E1同时表达GIPR和GLP-1 R。定量PCR分析表明，这两种受体的表达与BMP-2刺激后上调。阐明肠促胰岛素受体相关信号通路调控骨代谢的分子机制，将有助于开发有效的骨再生策略。

The purpose of this project is to explore the possible implementation of GPCR-B signaling in a new treatment method for bone diseases using an in vitro approach. Incretin receptors, GLP-1 receptor (GLP-1R) and GIP receptor (GIPR), belong to GPCR-B family and are suggested to play an important role in bone metabolism. The osteoblastic cell line, MC3T3-E1 was found to express both GIPR and GLP-1R. The quantitative PCR analysis showed that expression of both receptors was upregulated after stimulation with BMP-2. Determination of molecular mechanisms involved in regulation of bone metabolism by incretin receptors-related signaling will help to developed effective strategy for bone regeneration.