Study on establishment of the molecular biologic basewhich assumed mTOR for the periodontal regeneration.

mTOR牙周再生分子生物学基础建立的研究

• 批准号: 23792482
• 负责人: NAKAMURA Toshiaki
• 金额: $ 2.66万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792482/
• 关键词: 歯周病; 歯周組織再生; mTOR; FKBP12

In the periodontal regenerative therapy, we have considered that it is important to control host cells in periodontium and transplant cells to a state suitable for regeneration. In this study, it was intended that we established the molecular biologic base that controlled a cell to a state suitable for the periodontal regeneration by using the mammalian target of rapamycin (mTOR). Asa result, we had observed that FK506 enhanced BMP-2-induced-osteoblastic differentiation through smad-signaling pathway via FKBP12 on human cementoblasts (HCEM). Furthermore, in the induced pluripotent stem cells derived from a human gingival fibroblast (hGFiPS) culture system, we had observed that the colonization and gene expression patterns were altered in the addition of some mTOR inhibitors. This result may indicate that mTOR pathway plays a pivotal role in the hGFiPS.

在牙周再生治疗中，我们认为将牙周组织内的宿主细胞和移植细胞控制到适合再生的状态是非常重要的。在本研究中，我们打算利用哺乳动物雷帕霉素（mTOR）靶点，建立一种分子生物学基础，将细胞控制到适合牙周再生的状态。结果，我们观察到FK506通过smad信号通路通过FKBP12在人成骨水泥细胞（HCEM）上增强bmp -2诱导的成骨细胞分化。此外，在从人牙龈成纤维细胞（hGFiPS）培养系统中获得的诱导多能干细胞中，我们观察到在添加一些mTOR抑制剂后，定植和基因表达模式发生了改变。这一结果可能表明mTOR通路在hGFiPS中起着关键作用。

项目成果

Effects of FK506 on BMP-2 -induced osteo/cementoblastic differentiation in human cementoblast

FK506 对 BMP-2 诱导的人成牙骨质细胞成骨/成牙骨质细胞分化的影响

• 发表时间: 2012
• 作者: Toshiaki Nakamura;Kozue Hasegawa -Nakamura;Masae Kitagawa;Mutsumi Miyauchi;Takashi Takata and Kazuyuki Noguchi
• 通讯作者: Takashi Takata and Kazuyuki Noguchi

Effects of FK506 on BMP-2-induced osteo/cementoblastic differentiation in human cementoblast.

FK506 对 BMP-2 诱导的人成牙骨质细胞成骨/成牙骨质细胞分化的影响。

• 发表时间: 2012
• 作者: Toshiaki Nakamura;Kozue Hasegawa-Nakamura;Masae Kitagawa;Mutsumi Miyauchi;Takashi Takata and Kazuyuki Noguchi.
• 通讯作者: Takashi Takata and Kazuyuki Noguchi.

免疫抑制剤 FK506 がヒトセメント芽細胞のBMP-2 による細胞分化誘導に与える影響の検討

免疫抑制剂 FK506 对 BMP-2 诱导人成牙骨质细胞分化的影响的研究

• 发表时间: 2011
• 作者: 中村利明;中村梢;北川雅恵;宮内睦美;高田隆;野口和行
• 通讯作者: 野口和行