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細胞機能を可視化する新奇な超分子ナノ材料の光創製

光学创造新型超分子纳米材料以可视化细胞功能

基本信息

批准号：
11F01504
负责人：
BIJU Vasudevan Pillai (2013)
金额：
$ 1.28万
依托单位：
National Institute of Advanced Industrial Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

项目摘要

Our aim of this proposal is to develop a novel low-toxic, chemically stable, and highly-luminescent nanoparticles for single-molecule bio-sensing and -imaging using light as a resourceful tool. In this project, two types of novel fluorescent nanomaterials are prepared, which are fullerene-shelled CdSe-ZnS quantum dots and gold quantum clusters (Au25). Here, fullerene-shelled quantum dots are prepared by a photochemical method ; whereas, the quantum clusters are prepared by the alkaline reduction of gold ions within the pockets of bovine serum albumin. At first, a fullerene thiol molecule was synthesized by multi-step thermal chemical route. Subsequently, a monolayer of fullerene is prepared on the surface of QD by the tethering of the fullerene thiol to the ZnS surface through the S-S bonding. Finally, the fullerene thiol monolayer-protected QDs were immersed in excess fullerene thiol and photoactivated. We found the formation stable and thick fullerene shells on the surface of QDs, which is due to the 2+2 photocycloaddition reaction. Further, we characterized their photoluminescence properties at ensemble and single-molecule levels. The most interesting property of the fullerene-shelled QD is its ability to show suppressed blinking. While fullerene-shelled quantum dots show blinking suppression that is suitable for single-molecule bioimaging and solar energy harvesting ; bioconjugates of Au25 efficiently entered human cells and enabled us for obtaining NIR fluorescence images of live cells. The two types of fluorescent nanomaterials enabled us for establishing collaborations with four research groups in Japan. A part of this research is published in ACS Nano 6, 1601-1608, 2012.

我们的目标是开发一种新型的低毒、化学稳定、高度发光的纳米颗粒，用于单分子生物传感和成像，利用光作为一种丰富的工具。本项目制备了两种新型荧光纳米材料，分别是富勒烯壳CdSe-ZnS量子点和金量子团簇（Au25）。在这里，富勒烯壳量子点是通过光化学方法制备的；然而，量子团簇是通过在牛血清白蛋白的口袋中碱性还原金离子制备的。首先，采用多步热化学方法合成了富勒烯硫醇分子。随后，通过S-S键将富勒烯硫醇系聚到ZnS表面，在QD表面制备了单层富勒烯。最后，将单层保护的量子点浸入过量的富勒烯硫醇中进行光活化。我们发现在量子点表面形成稳定且厚实的富勒烯壳层，这是由于2+2光环加成反应。进一步，我们在系综和单分子水平上表征了它们的光致发光特性。富勒烯壳量子点最有趣的特性是它具有抑制闪烁的能力。而富勒烯壳量子点则表现出适合单分子生物成像和太阳能收集的闪烁抑制；Au25的生物偶联物有效地进入人细胞，使我们能够获得活细胞的近红外荧光图像。这两种荧光纳米材料使我们能够与日本的四个研究小组建立合作关系。这项研究的一部分发表在ACS Nano 6, 1601-1608, 2012。