Why is the photosynthetic CO2-fixing enzyme, Rubisco, so inefficient? Dissection of the catalytic chemistry by computational simulation and experimental testing.

为什么光合二氧化碳固定酶 Rubisco 的效率如此低下？

• 批准号: LP0347461
• 负责人: Prof Jill Gready
• 金额: $ 24.09万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-03-06 至 2005-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0347461
• 关键词: Why; photosynthetic; CO2; fixing; enzyme

Fixation of CO2 by the enzyme Rubisco during photosynthesis produces organic compounds which feed all life. Despite this critical role, Rubisco catalyses its reaction sluggishly and, worse, discriminates poorly between CO2 and O2, leading to useless products. Our combined expertise equips us to analyse Rubisco's mechanism using quantum-chemical methods and then test predictions experimentally. We will capitalise on our previous successful studies of Rubisco by addressing emergent issues which are the keys to understanding catalytic efficiency and CO2/O2 selectivity: the roles of a carbamylated lysine; the way CO2 addition is rendered irreversible; and the spin inversion inherent in O2 addition.

在光合作用过程中，Rubisco酶对CO2的固定产生了有机化合物，这些有机化合物为所有生命提供了食物。尽管Rubisco具有这种关键作用，但它催化反应的速度很慢，更糟糕的是，它对CO2和O2的区分能力很差，导致产生无用的产物。我们的综合专业知识使我们能够使用量子化学方法分析Rubisco的机制，然后通过实验测试预测。我们将利用我们以前的成功研究Rubisco解决紧急问题，这是理解催化效率和CO2/O2选择性的关键：氨甲酰化赖氨酸的作用; CO2加成的方式是不可逆的;和自旋反转固有的O2加成。