Development ofa novel method for analyzing various DNA methylation

开发分析各种 DNA 甲基化的新方法

• 批准号: 23750083
• 负责人: SUZUKI Hirokazu
• 金额: $ 3万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23750083/
• 关键词: メチル化塩基; メチル化部位分析; 抗体; 分析方法

Microbes harbor various methylated DNA species, such as 5-methylcytosine, N6-methyladenine, and N4-methylcytosine, which are produced by DNA restriction-modification systems. Aiming at the development of a novel method for widely analyzing DNA methylation sites, I designed immunological methods to analyze N6-methyladenine sites arising from type I restriction-modification systems (immunoprecipitation/PCR, methylated plasmid library/HPLC, methylated plasmid library/western blot) and evaluated them. Detailed analysis suggested that the method “methylated plasmid library/western blot” was effective for analyzing type I methylation sites.

微生物含有多种甲基化 DNA 种类，例如 5-甲基胞嘧啶、N6-甲基腺嘌呤和 N4-甲基胞嘧啶，它们是由 DNA 限制性修饰系统产生的。为了开发一种广泛分析DNA甲基化位点的新方法，我设计了免疫学方法来分析I型限制性修饰系统（免疫沉淀/PCR、甲基化质粒文库/HPLC、甲基化质粒文库/蛋白质印迹）产生的N6-甲基腺嘌呤位点并对其进行评估。详细分析表明“甲基化质粒文库/蛋白质印迹”方法对于分析I型甲基化位点是有效的。

项目成果

In: Methylation - From DNA, RNA and Histones to Diseases and Treatment (edited by Anica Dricu), InTech, Rijeka, Croatia

见：甲基化 - 从 DNA、RNA 和组蛋白到疾病和治疗（由 Anica Dricu 编辑），InTech，里耶卡，克罗地亚

• 发表时间: 2012
• 作者: M. Hara;J. Inukai;B. Bae;T. Hoshi,K. Miyatake;M. Uchida;H. Uchida;M.Wtanabe;Hirokazu Suzuki
• 通讯作者: Hirokazu Suzuki

Host-mimicking strategies in DNA methylation for improved bacterial transformation. In: Methylation – From DNA, RNA and Histones to Diseases and Treatment (edited by Anica Dricu)

DNA 甲基化中的宿主模仿策略可改善细菌转化。见：甲基化 – 从 DNA、RNA 和组蛋白到疾病和治疗（由 Anica Dricu 编辑）。

• 发表时间: 2012
• 作者: 早坂和将;福本晃造;小谷研悟;中沢 浩;Hirokazu Suzuki
• 通讯作者: Hirokazu Suzuki