Study for elucidating the mechanism for the specific degradation of spider silk by ultraviolet rays

阐明紫外线特异性降解蜘蛛丝机理的研究

• 批准号: 23750133
• 负责人: MATSUHIRA Takashi
• 金额: $ 2万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23750133/
• 关键词: 生体関連高分子; クモの糸; 紫外線; タンパク質; 分子量; 電気泳動; 絹糸; SDS PAGE

Previously, Osaki et al. showed that the silk of the Nephila clavata spider was more resistant to UV irradiation than that of the Bombyx mori silkworm. In the present study, we focused on the difference in UV resistivity between silkworm silk and spider silk from the viewpoint of molecular weight. Generally, molecular weight is determined using gel-electrophoresis method after denaturing proteins by sodium dodecyl sulfate (SDS PAGE). Here, the molecular weight of spider silk protein was determined to be 272 kDa, which is smaller than that (350 kDa) of silkworm silk using SDS PAGE.We tried to change the method for denaturing spider silk to realize the native condition. The molecular weight of native spider silk protein was determined to be about 600-700 kDa, which was much larger than that of silkworm silk. Such a large molecular weight may be ascribed to cross-linkages of protein molecules with a molecular weight of 272 kDa.

此前，Osaki等人。结果表明，棒形新丝虫的丝质比家蚕的丝质具有更强的抗紫外线能力。本研究从分子质量的角度对蚕丝和蜘蛛丝的抗紫外线性能差异进行了研究。通常，蛋白质经十二烷基硫酸钠(十二烷基硫酸钠)变性后，用凝胶电泳法测定其相对分子质量。本文用SDS-PAGE测定了蜘蛛丝蛋白的相对分子质量为272 kDa，小于用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法测定的蚕丝蛋白的相对分子质量350 kDa。天然蜘蛛丝蛋白的相对分子质量约为600-700 kDa，远大于蚕丝的相对分子质量。如此大的相对分子质量可以归因于分子量为272 kDa的蛋白质分子的交联性。

项目成果

天然のクモ糸の分子量は一体いくらなのか?

天然蜘蛛丝的分子量是多少？

• 发表时间: 2013
• 作者: Yoshihiro Kikkawa;Masato Fukuda;Tomoya Kimura;Ayumi Kashiwada;Kiyomi Matsuda;Masatoshi Kanesato;Masahisa Wada;Tadayuki Imanaka;Takeshi Tanaka;大崎茂芳,松平崇
• 通讯作者: 大崎茂芳,松平崇

Effects of UV irradiation on the molecular weight of spider silk

紫外辐射对蜘蛛丝分子量的影响

• DOI: 10.1038/pj.2013.41
• 发表时间: 2013
• 期刊: Polymer Journal
• 影响因子: 2.8
• 作者: Takashi Matsuhira;Keizo Yamamoto and Shigeyoshi Osaki
• 通讯作者: Keizo Yamamoto and Shigeyoshi Osaki

Effects of UV Rays upon Molecular Weight of Spider Silk

紫外线对蜘蛛丝分子量的影响

• 作者: 松平崇;大﨑茂芳;大﨑茂芳;大﨑茂芳;大﨑茂芳;Shigeyoshi Osaki;Takashi Matsuhira & Shigeyoshi Osaki
• 通讯作者: Takashi Matsuhira & Shigeyoshi Osaki

高分子量のクモの糸タンパク

高分子量蜘蛛丝蛋白

• 发表时间: 2011
• 作者: Takeshi Yamamoto;Yuto Akai;Yuuya Nagata;and Michinori Suginome;松平崇,大崎茂芳
• 通讯作者: 松平崇,大崎茂芳

クモ糸のタンパク質の分子量

蜘蛛丝蛋白的分子量

• 发表时间: 2011
• 作者: K. Namba;A. Mera;A. Osawa;E. Sakuda;N. Kitamura and K. Tanino;松平崇,大崎茂芳
• 通讯作者: 松平崇,大崎茂芳