Development of porphyrin photosensitizer for photodynamic therapy

光动力治疗用卟啉光敏剂的研制

• 批准号: 23750186
• 负责人: HIRAKAWA Kazutaka
• 金额: $ 2.83万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23750186/
• 关键词: 光線力学的療法; 光増感剤; ポルフィリン; 電子移動; 一重項酸素; タンパク質損傷; トリプトファン; 酸化反応; アミノ酸分解; がん治療; 酵素活性阻害; 光増感反応; DNA; 細胞毒性

The purpose of this study is the development of novel photosensitizer for photodynamic therapy (PDT), which is a less invasive treatment of cancer. In general, porphyins were used as the PDT photosensitizer. Administered porphyrins can generate singlet oxygen to photosensitize biomolecules oxidation. However, since the oxygen concentration is relatively low in tumor, the PDT effect in tumor is restricted. In this study, P(V)porphyrins were used as photosensitizers. P(V)porphyrins induced the oxidation of amino acid residue in a water soluble protein, a target model biomolecule, during visible-light irradiation. It was confirmed that the mechanism of protein photodamage by these porphyrins is singlet oxygen generation and photo-induced electron transfer. For this experiment, the novel analysis method to detect the protein damage was developed. Further, the novel porphyrin photosensitizers were synthesized and their activities were demonstrated.

这项研究的目的是开发新的光动力疗法光敏剂（PDT），这是对癌症的侵入性较低的治疗方法。通常，卟啉被用作PDT光敏剂。施用的卟啉可以产生单线氧，以使生物分子氧化光敏。但是，由于氧气浓度的肿瘤相对较低，因此肿瘤中的PDT效应受到限制。在这项研究中，P（V）卟啉被用作光敏剂。 p（v）卟啉在可见光照射期间诱导水溶性蛋白（靶模型生物分子）中氨基酸残基的氧化。已经证实，这些卟啉受到蛋白质光损伤的机理是单线氧的产生和光诱导的电子转移。对于此实验，开发了检测蛋白质损伤的新分析方法。此外，合成了新型的卟啉光敏剂，并证明了它们的活动。

项目成果

Activity control of singlet oxygen generation by porphyrin photosensitizers for photodynamic therapy through interaction with DNA

通过与 DNA 相互作用进行光动力治疗的卟啉光敏剂对单线态氧产生的活性控制

• 发表时间: 2013
• 作者: Kazutaka Hirakawa;Mari Harada;Yoshinobu Nishimura;Tatsuo Arai;Yoshio Nosaka;Shigetoshi Okazaki
• 通讯作者: Shigetoshi Okazaki

DNA認識で発現する水溶性ポルフィリン光増感剤の一重項酸素生成活性

DNA识别表达的水溶性卟啉光敏剂的单线态氧生成活性

• 发表时间: 2012
• 作者: T. Sakai;H. Seo;S. Aihara;M. Kubota;N. Egami;K. Mori;T. Fukuda;K. Hatano;N. Kamata;平川和貴,原田万理,岡崎茂俊
• 通讯作者: 平川和貴,原田万理,岡崎茂俊

Control of singlet oxygen generating activity of meso-pyrenylporphyrin through interaction with DNA

通过与 DNA 相互作用控制内消旋芘基卟啉的单线态氧生成活性

• 发表时间: 2012
• 作者: Kazutaka Hirakawa;Mari Harada;Shigetoshi Okazaki;Yoshinobu Nishimura;Tatsuo. Arai;Yoshio Nosaka
• 通讯作者: Yoshio Nosaka

In hope of going over the present Clinical PD and PDT

希望能够回顾一下目前的临床PD和PDT

• 发表时间: 2011
• 作者: Kazutaka Hirakawa;Hiroshi Segawa;Toru Hirano;Shigetoshi Okazaki;and Yoshio Nosaka et al. ed. by Norio Miyoshi and Roy H. Pottier
• 通讯作者: and Yoshio Nosaka et al. ed. by Norio Miyoshi and Roy H. Pottier

Photo-oxidation of human serum albumin by the singlet excited state of water-soluble P(V)porphyrin

水溶性 P(V) 卟啉单线态激发对人血清白蛋白的光氧化

• 发表时间: 2011
• 作者: Kazutaka Hirakawa;Hiroki Yamaguchi;Hironubu Umemoto;Shigetoshi Okazaki;Yoshinobu Nishimura;Tatsuo Arai;Hiroshi Segawa
• 通讯作者: Hiroshi Segawa