development of functional nucleic acids toward fluorescense imaging of chemical transmitter dynamics

我们展示了荧光适体在细胞表面细胞外化学递质的分子传感中的应用。我们开发了一种荧光适体传感器，可以固定在细胞表面，用于分析细胞外事件。本方法实现了腺嘌呤核苷酸（Ade）荧光适配体在细胞表面的简单、高效的固定，并实现了活细胞Ade外排的荧光可视化。

We demonstrated an application of fluorescent aptamer for molecular sensing of extracellular chemical transmitters dynamics on cell surface. We developed a fluorescent aptamer sensor that could be immobilized on cell surface for the analysis of extracellular events. Our approach enabled an easy and efficient immobilization of the fluorescent adenine nucleotide(Ade) aptamer on cell surface, and realized the fluorescence visualization of Ade efflux from living cell.