The Microbial Community Structure of Relict Niter-beds Previously Used for Saltpeter Production

硝石生产残余硝床的微生物群落结构

• 批准号: 23760512
• 负责人: NARIHIRO Takashi
• 金额: $ 2.83万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23760512/
• 关键词: 環境微生物学; 微生物生態学; 硝化反応; 土壌環境; 微生物生態; 環境生態; 微生物分類; 国際情報交換; アメリカ合衆国

The saltpeter had been produced by using niter-bed process formed under the floor of gassho-style house in a Historic Village of Shirakawa-go and Gokayama. The diversity of the nitrifying microbes in such relict niter-bed soil remains unclear. Here, the microbial assemblages of relict niter-bed soils were performed by pyrosequencing analysis targeting 16S rRNA gene and ammonia monooxygenase gene (amoA). The 16S rRNA gene analyses revealed that members of the phyla Proteobacteria, Actinobacteria, and Bacteroidetes were major microbial constituents, and principal coordinate analysis showed that soil ion contents were critical factors for the communities. The amoA pyrotag analysis revealed that Nitrosospira and "Ca. Nitrososphaera" predominated, and soil pH and organic carbon content were important factors for the ecological niche of nitrifying microbes. These results suggest that these organisms may play roles in ammonia-oxidation in relict niter-bed soil ecosystems.

硝石是通过在白川乡和五箇山历史村落的合掌式房屋地板下形成的硝石床工艺生产的。这种残余硝床土壤中硝化微生物的多样性仍不清楚。在这里，通过针对 16S rRNA 基因和氨单加氧酶基因 (amoA) 的焦磷酸测序分析对残余硝基土壤的微生物组合进行了分析。 16S rRNA基因分析表明，变形菌门、放线菌门和拟杆菌门的成员是主要微生物成分，主坐标分析表明土壤离子含量是群落的关键因素。 amoA热标记分析表明，硝化微生物以亚硝化螺菌和“亚硝化菌”为主，土壤pH和有机碳含量是影响硝化微生物生态位的重要因素。这些结果表明这些生物可能在残硝床土壤生态系统的氨氧化中发挥作用。