Artificial control of bone mechanical functions usingmagnesium as a biological trace metal

利用体内(动物模型)和体外(磷灰石合成)系统研究了镁作为生物痕量金属对骨磷灰石择优取向的影响。随着低镁饮食的摄入，大鼠长骨中磷灰石c轴择优取向的程度发生了显着变化。在湿法在玻璃磷灰石合成中，尽管作为磷灰石形成模板的胶原排列是恒定的，但择优取向的程度随着镁离子/钙离子比(Mg2/Ca2)的变化而变化。定向度在特定的镁/钙比值处达到峰值，峰值与大鼠股骨相当。结果表明，生物痕量金属影响胶原与磷灰石c轴之间的外延程度，最终控制主导骨力学功能的磷灰石取向。证明了生物痕量金属在控制磷灰石取向和由此产生的机械功能方面的潜力。

The effects of magnesium as a biological trace metal on the preferential orientation of bone apatite were elucidated using in vivo (animal model)and in vitro(apatite synthesis) systems. Degree of the preferential apatite c-axis orientation along the long axis in a rat long bone significantly changed along with the administration of a magnesium-deficient diet. In the in vitroapatite synthesis through a wet process, the degree of the preferential apatite orientation varied as a function of the magnesium ion/calcium ion ratio (Mg2+/Ca2+) despite the collagen alignment used as a template for the apatite formation was constant. The orientation degree peaked at the specific Mg2+/Ca2+; the peak value was comparable to that in the rat femur. The results indicate that the biological trace metals affect the degree of epitaxy between collagen and apatite c-axis, and finally control the apatite orientation which predominate the bone mechanical functions. The potential of the biological trace metals on the control of the apatite orientation and the resultant mechanical functions was demonstrated.