Development of capillary-like network for creating three-dimensional tissues

开发用于创建三维组织的毛细管状网络

• 批准号: 23760752
• 负责人: TAKEI Takayuki
• 金额: $ 2.91万
• 依托单位: Kagoshima University (2012)Kyushu University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23760752/
• 关键词: 毛細血管; ヒドロゲル; ファイバー; 再生医療

Poly(methyl methacrylate)(PMMA)microfibers of approximately 10μm in diameter were prepared using wet spinning technique. The microfibers were embedded in agarose gel and dissolved specifically, resulting in formation of capillary-like network in the gel. The gel was freeze-dried and hepatoblastomawas seeded into the sponge-like gel. Then, the sponge was coated with agarose gel. Culture medium was perfused into the capillary-like network in the sponge. Albumin productivity of the sponge with perfusion of medium was higher than that without medium perfusion.

采用湿法纺丝技术制备了直径约10μm的聚甲基丙烯酸甲酯超细纤维。微纤维被包埋在琼脂糖凝胶中，并被特异性溶解，从而在凝胶中形成毛细管状网络。冻干凝胶，将肝母细胞种植到海绵状凝胶中。然后，用琼脂糖凝胶包覆海绵。将培养基灌入海绵中的毛细血管样网络中。培养基灌注组海绵的白蛋白产量高于未灌注组。

项目成果

Injectable and biodegradable sugar beet pectin-based hydrogels for biomedical applications

用于生物医学应用的可注射且可生物降解的甜菜果胶基水凝胶

• 发表时间: 2012
• 作者: Takayuki Takei;Kotaro Sugihara;Hiroyuki Ijima and Koei Kawakami
• 通讯作者: Hiroyuki Ijima and Koei Kawakami

Fabrication of capillary-like network in a matrix of water-soluble polymer using poly(methyl methacrylate) microfibers

使用聚甲基丙烯酸甲酯微纤维在水溶性聚合物基质中制造毛细管状网络

• DOI: 10.3109/10731199.2011.592492
• 发表时间: 2012
• 期刊: Artificial Cells, Blood Substitutes, and Biotechnology
• 作者: Takayuki Takei;Naoya Kishihara;Hiroyuki Ijima and Koei Kawakami
• 通讯作者: Hiroyuki Ijima and Koei Kawakami