Development of new method in structural analysis for larger proteinsusing nuclear magnetic resonances
Development of new method in structural analysis for larger proteinsusing nuclear magnetic resonances
批准号:
23770111
负责人:
MIYANOIRI Yohei
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
We have developed new SAIL aromatic amino acids which are optimized relaxation properties in aromatic ring and successfully observed extremely well-separated aromatic CH cross peaks in TROSY experiment, even for an 82kDa E. colimalate synthase G (MSG) protein. Using our new SAILaromatic CH TROSY method, we could clearly assign many NOE signals among aromatic CH, methyl and amide signals which are very important for determining the precise structure of large molecular proteins.
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SAIL aromatic CH TROSY to investigate larger proteins
SAIL 芳香族 CH TROSY 用于研究更大的蛋白质
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Miyanoiri Y, Takeda M, Ono AM, Okuma K, Terauchi T, Kainosho M]
通讯作者:
Kainosho M
名古屋大学大学院理学研究科附属構造生物学研究センター 甲斐荘グループ
名古屋大学研究生院研究生院结构生物学研究中心快搜组
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
DOI:
10.1007/978-94-007-4954-2_5
发表时间:
2012-01-01
期刊:
ISOTOPE LABELING IN BIOMOLECULAR NMR
影响因子:
--
作者:
[Miyanoiri, Yohei, Takeda, Mitsuhiro, Kainosho, Masatsune]
通讯作者:
Kainosho, Masatsune
Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel
真菌瞬时受体电位(TRP)通道多模式调节的分子基础
DOI:
10.1074/jbc.m112.434795
发表时间:
2013
期刊:
Journal of Biological Chemistry
影响因子:
4.8
作者:
[Ihara M,Hamamoto S, Miyanoiri Y, Takeda M, Kainosho M, Yabe I, Uozumi N, Yamashita A]
通讯作者:
Yamashita A
SAIL法による巨大蛋白質の新規立体構造解析法の開発
开发使用SAIL方法分析大蛋白质三维结构的新方法
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Bennett TL, Kraft SM, Reaves BJ, Mima J, O'Brien KM, Starai VJ, 宮ノ入洋平]
通讯作者:
宮ノ入洋平
共 8 条
Development of the new method for dynamics analysis in large molecular protein-protein complex using nuclear magnetic resonance
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批准号:25840021
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.83万
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财政年份:2013
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负责人:MIYANOIRI Yohei
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依托单位:
海外基金