Development of new method in structural analysis for larger proteinsusing nuclear magnetic resonances

我们开发了新的SAIL芳香氨基酸，优化了芳香环的弛豫特性，并在TROSY实验中成功地观察到分离非常好的芳香CH交叉峰，即使是82kDa的E. colcolalate合成酶G （MSG）蛋白。利用SAILaromatic CH TROSY方法，我们可以在芳香CH、甲基和酰胺信号中清晰地划分出许多NOE信号，这对确定大分子蛋白质的精确结构非常重要。

We have developed new SAIL aromatic amino acids which are optimized relaxation properties in aromatic ring and successfully observed extremely well-separated aromatic CH cross peaks in TROSY experiment, even for an 82kDa E. colimalate synthase G (MSG) protein. Using our new SAILaromatic CH TROSY method, we could clearly assign many NOE signals among aromatic CH, methyl and amide signals which are very important for determining the precise structure of large molecular proteins.