Structural study of slective cargo transport by unconventional myosins

非常规肌球蛋白选择​​性货物运输的结构研究

• 批准号: 23770119
• 负责人: HIRANO Yoshinori
• 金额: $ 3万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770119/
• 关键词: X線結晶構造解析; 分子認識; 積荷輸送; ミオシン; 微小管; 細胞内輸送; 積荷認識; 軸索ガイダンス; モータータンパク質; 結晶構造解析; 分子複合体; 積み荷認識

The Cargo transportation by myosins is essential for proper cell phenomena. We peformed a series of structural and biochemicalstudies of the myosin-X tail domain , consisting of MyTH4 and FERM domains in complex with its specific cargo, a netrin receptor DCC (deleted in colorectal cancer). As the result, we found an unexpected binding mode of the DCC peptide, which is distinct from previously reported associations found in radixin/adhesion molecule complexes.Moreover, we revealed direct interactions between the MyTH4-FERM domain and tubulin C-terminal acidic tails, and identified a positively charged patch of the MyTH4 domain as a tubulin binding region. We also demonstrated that both DCC and integrin bindings interfere with microtubule binding and that DCC binding interferes with integrin binding. Our results provide the molecular basis by which myosin-X facilitates alternative dual binding to cargos and microtubules.

肌球蛋白的货物运输对于正常的细胞现象是必不可少的。我们对肌球蛋白-X尾部结构域进行了一系列结构和生化研究，该结构域由MyTH 4和FERM结构域及其特异性负载物netrin受体DCC（在结直肠癌中缺失）组成。结果，我们发现了一个意想不到的结合模式的DCC肽，这是不同于以前报道的协会发现radixin/adhesion molecule complexes. Further，我们揭示了直接的相互作用之间的MyTH 4-FERM域和微管蛋白C-末端的酸性tails. And确定了一个带正电荷的补丁的MyTH 4域作为微管蛋白的结合区域。我们还证明了DCC和整合素结合都干扰微管结合，DCC结合干扰整合素结合。我们的研究结果提供了肌球蛋白-X促进货物和微管的替代性双重结合的分子基础。

项目成果

Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain.

• DOI: 10.1038/emboj.2011.177
• 发表时间: 2011-06-03
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Hirano, Yoshinori;Hatano, Taiki;Takahashi, Aya;Toriyama, Michinori;Inagaki, Naoyuki;Hakoshima, Toshio
• 通讯作者: Hakoshima, Toshio

Structural and functional analyses of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance

参与氧化应激耐受的酵母 N-乙酰转移酶 Mpr1 的结构和功能分析

• 发表时间: 2013
• 作者: Hirano,Y.;Hatano,T.;Takahashi,A.;Toriyama,M.;Inagaki,N. Hakoshima,T;Yoshinori Hirano;那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史;那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史;那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
• 通讯作者: 那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史

酸化のストレスから酵母を守るカギの酵素「Mpr1」の構造と反応機構を解明

阐明保护酵母免受氧化应激的关键酶 Mpr1 的结构和反应机制

Mechanism of high-affinity abscisic acid binding to PYL9/RCAR1

• DOI: 10.1111/gtc.12140
• 发表时间: 2014-05-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Nakagawa, Masahiro;Kagiyama, Megumi;Hakoshima, Toshio
• 通讯作者: Hakoshima, Toshio

Structures of D14 and D14L in the strigolactone and karrikin signaling pathways

• DOI: 10.1111/gtc.12025
• 发表时间: 2013-02-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Kagiyama, Megumi;Hirano, Yoshinori;Hakoshima, Toshio
• 通讯作者: Hakoshima, Toshio