X-ray structure analysis of rare sugar producible enzymes aiming for elucidation of novel isomerization mechanism

不动杆菌新异构酶L-核糖异构酶的X射线结构(L-RI)。L-RI可以催化L-核糖和L-核酮糖之间的可逆异构化反应，是一种可能的稀糖生产酶。在结构分析中，L-RI显示了二聚体的形成。L-RI的单体结构与大肠杆菌O157：H7的D-赖糖异构酶相似。L-RI在活性中心保留了一个金属离子和三个组氨酸。两种谷氨酸可能是催化残基。

X-ray structure of a novel isomerase, L-ribose isomerase from Acinetobacter sp. (L-RI) was determined. L-RI can catalyze reversible isomerization between L-ribose and L-ribulose, and is a plausible enzyme for rare sugar production. In the structure analysis, L-RI showed the dimer formation. The monomer structure of L-RI is similar to that of D-lyxose isomerase from Escherichia coli O157:H7. L-RI retains a metal ion in the active site with three histidines. Two glutamates are putative catalytic residues.