Heme-sensing ChrS/ChrA system from Corynebacterium diphtheriae

为了感染宿主并在宿主中增殖，病原菌使用铁获取系统优先获取来自宿主Hb的铁。白喉棒状杆菌（Corynebacterium diphtheriae）是白喉的病原体，其传导铁/血红素依赖的调节ChrS/ChrA系统。在本研究中，ChrS蛋白溶解和纯化从大肠杆菌的膜过度生产，然后纳入纳米盘。重组ChrS蛋白通过第一跨膜区的His 21直接特异性识别血红素，并通过血红素依赖性自磷酸化和磷酸化转移到ChrA。

To infect and proliferate in the host, pathogenic bacteria preferentially acquire iron derived from host Hb using iron-acquisition systems. Corynebacterium diphtheriae, a pathogen of diphtheria, conducts iron/heme-dependent regulatory ChrS/ChrA systems. In the present study, the ChrS protein was solubilized and purified from the membranes of an Escherichia coli overproducer, and then incorporated into Nanodiscs. The reconstituted ChrS protein exhibited direct and specific heme recognition via His21 in its first transmembrane region, hemedependent autophosphorylation and phosphoryltransfer to ChrA.