Development of novel type of flat lipid membrane for observation of complex formation process and structural analysis of divisome

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Many of membrane proteins are recruited at cell division site and drive the E. coli cell division. The membrane proteins interact with each other and forms huge protein complex named divisome. At present, fundamental mechanism of divisome formation and structure of the complex on cell membrane has not been clear. In this study, we developed a new model membrane, which can analyze a structure and functions of huge membrane proteins complex, and tried to reveal molecular mechanism of E. coli cell division. We succeeded in flat lipid membrane at liquid-air interface using E. coli extracted lipid. We prepared some GFP fusion membrane proteins, which drive cell division, and reconstituted into the model membrane. At the end of this study period, we could not find membrane protein into the model membrane. Recently, we developed a new method for preparation of cell membrane sheet derived from cultured cell. We will keep trying to analyze membrane protein complex by using the model membrane.