Neutron Crystallographic study of hydration structures of DNA and its fluctuation

DNA水合结构及其波动的中子晶体学研究

• 批准号: 23770176
• 负责人: CHATAKE Toshiyuki
• 金额: $ 2.75万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770176/
• 关键词: 水和構造; 揺らぎ; DNA; 中性子

For the purpose of revealing structure of DNA hydration including structural fluctuation, neutron and X-ray crystallographic studies were carried out. (1) Deuterated Z-DNA d(CGCGCG) could be produced. (2) Large single crystals of Z-DNA d(CGCGCG) were obtained from H2O solution, and TOF neutron experiment was performed using pulsed neutron source. Neutron diffraction spots were detected up to 1.6 -1.8 Angstrom. Protocol for refinements was tested using neutron data sets of protein crystals. X-ray crystallographic analyses were also carried out complementarily, as the result, Z-DNA structures at high and low concentrations of metal salts were determined. Each of these structures had specific features, suggesting that these crystals would be dominant candidates for neutron crystallographic studies.

为了揭示DNA水化的结构，包括结构涨落，进行了中子和X射线晶体学研究。(1)可产生氘代Z-DNAd（CGCGCG）。(2)从水溶液中获得了Z-DNAd（CGCGCG）大单晶，并利用脉冲中子源进行了飞行时间中子实验。在1.6 - 1.8埃范围内检测到中子衍射斑点。使用蛋白质晶体的中子数据集进行了测试的改进协议。X射线晶体学分析也进行了补充，作为结果，Z-DNA结构在高和低浓度的金属盐的测定。这些结构中的每一个都有特定的特征，这表明这些晶体将是中子晶体学研究的主要候选者。

项目成果

納豆由来水溶性ビタミンK2の研究

纳豆水溶性维生素K2的研究

• 发表时间: 2014
• 作者: 茶竹俊行(発表者);柳澤泰任;松尾龍人;藤原悟;森本幸生
• 通讯作者: 森本幸生

生体分子の中性子単結晶解析(第一回)蛋白質中性子結晶学の基礎

生物分子的中子单晶分析（第一节）蛋白质中子晶体学基础

• 发表时间: 2013
• 期刊: 日本中性子科学会誌
• 作者: 茶竹俊行;森本幸生
• 通讯作者: 森本幸生

Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl2 and CaCl2

• DOI: 10.1016/j.jinorgbio.2013.03.004
• 发表时间: 2013-07-01
• 期刊: JOURNAL OF INORGANIC BIOCHEMISTRY
• 影响因子: 3.9
• 作者: Chatake, Toshiyuki;Sunami, Tomoko
• 通讯作者: Sunami, Tomoko

X-ray structure determination and deuteration of nattokinase.

X射线结构的确定和nattokinase的侵蚀。

• DOI: 10.1107/s0909049513020700
• 发表时间: 2013-11
• 期刊: Journal of synchrotron radiation
• 影响因子: 2.5
• 作者: Yanagisawa Y;Chatake T;Naito S;Ohsugi T;Yatagai C;Sumi H;Kawaguchi A;Chiba-Kamosida K;Ogawa M;Adachi T;Morimoto Y
• 通讯作者: Morimoto Y

高塩状態で誘導されるDNA会合の研究

高盐条件诱导DNA缔合的研究

• 发表时间: 2012
• 作者: 樋口芳樹; 茶竹俊行; 横山武司; 山田太郎 (代表発表者: 樋口芳樹);白崎 善隆;茶竹俊行
• 通讯作者: 茶竹俊行