Cloning of wheat vernalization gene Vrn-D4 and its functional analysis

小麦春化基因Vrn-D4的克隆及其功能分析

• 批准号: 23780005
• 负责人: NISHIDA Hidetaka
• 金额: $ 2.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780005/
• 关键词: コムギ; 春播性遺伝子; Vrn-D4; クローニング; VIL-D1

To dissect the wheat vernalization mechanism, molecular cloning of all vernalization genes and their functional analyses need to be conducted. In this study, we focused on the vernalization gene Vrn-D4. We constructed the high density genetic map around the Vrn-D4 locus, which narrowed down the Vrn-D4 region to the 0.09cM interval. By referring the genomic information on the Brachypodium corresponding region, we selected Vrn-D4 candidate genes. However, no significant variation was found.

为了深入研究小麦春化机理，需要对所有春化基因进行分子克隆和功能分析。本研究以春化基因Vrn-D4为研究对象。以Vrn-D4位点为中心构建了高密度遗传图谱，将Vrn-D4区域缩小到0.09cM。通过参考短柄草属植物相应区域的基因组信息，我们筛选出了Vrn-D4候选基因。然而，没有发现显著的变化。

项目成果

Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat.

• DOI: 10.1007/s00438-013-0788-y
• 发表时间: 2014-02
• 期刊: Molecular genetics and genomics : MGG
• 作者: Kippes N;Zhu J;Chen A;Vanzetti L;Lukaszewski A;Nishida H;Kato K;Dvorak J;Dubcovsky J
• 通讯作者: Dubcovsky J