Study of relationship between antioxidant activity of soybean flavonoids and chilling stress, and their related gene

大豆黄酮类化合物抗氧化活性与低温胁迫关系及相关基因研究

• 批准号: 23780009
• 负责人: TODA Kyoko
• 金额: $ 2.75万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780009/
• 关键词: ダイズ; 低温ストレス耐性; フラボノイド; 大豆; 形質転換; 高速液体クロマトグラフィー（HPLC）

Our studies and others have suggested that soybean F3`H gene corresponding to the T locus, which controls pubescence color, is related to chilling stress in soybean. The aim of this study was to clarify the relationship between F3`H and chilling stress using transformed soybean, and its mechanism by morphological study and analyzing flavonoids. A transformant was obtained and gene expression was confirmed by RT-PCR and HPLC analyses of flavonoids. Morphological study and scavenging activity of the DPPH radical indicated that F3`H is localized to the tonoplast in the hilum and funiculus of the immature seed coat, affecting biosynthesis of flavonoid and antioxidant properties in those tissues.

我们的研究和其他人的研究表明，大豆F3`H基因对应的T位点，控制毛的颜色，在大豆中与低温胁迫有关。本研究以转基因大豆为材料，通过形态学研究和黄酮类化合物分析，阐明F3`H与低温胁迫的关系及其作用机制。通过RT-PCR和黄酮类化合物的HPLC分析，获得了一个表达载体，并证实了基因的表达。形态学研究和DPPH自由基清除活性表明，F3`H定位于未成熟种皮的种脐和柄的液泡膜中，影响这些组织中类黄酮的生物合成和抗氧化性质。

项目成果

Localization and physiological function of flavonoid 3’-hydroxylase in soybean

大豆类黄酮3-羟化酶的定位及生理功能

• 发表时间: 2014
• 作者: Kyoko Toda;Haruko Kuroiwa;Kalaiselvi Senthil;Ryoji Takahashi
• 通讯作者: Ryoji Takahashi

Localization and possible role in chilling tolerance of flavonoid 3'-hydroxylase in soybean

大豆类黄酮 3-羟化酶的定位及其在耐冷性中的可能作用

• 发表时间: 2014
• 作者: 戸田恭子;黒岩晴子;Kalaiselvi Senthil;高橋良二
• 通讯作者: 高橋良二

The soybean F3'H protein is localized to the tonoplast in the seed coat hilum

大豆 F3H 蛋白定位于种皮门的液泡膜

• DOI: 10.1007/s00425-012-1590-5
• 发表时间: 2012
• 期刊: Planta
• 影响因子: 4.3
• 作者: Toda;K.;et al
• 通讯作者: et al