Structural and functional analyses of capsid protein and RNA polymerase in the candidate virus for honeybee colony collapse disorder

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Colony Collapse Disorder (CCD) is a serious problem threatening the health of honeybees and the economic stability of commercial beekeeping and pollination operations in the world. In this study, we focused on Israeli Acute Paralysis Virus (IAPV), which is considered as a candidate reason for CCD, and challenged to investigate the structure and function of the capsid protein and the RNA polymerase in IAPV. Recombinant proteins of the capsid proteins were expressed, solubilized and purified by combinational expression of 2 or 3 kinds of subunit proteins. Next, we found 2 proteins, which seemed to interact with these recombinant capsid protein subunits, from honeybee brains and guts. Two of 6 partial recombinant proteins of RNA polymerase was successful for expression and solubilization. We will try to isolate the proteins in honeybee tissues which interact with these recombinant proteins.