Structural and functional analyses of capsid protein and RNA polymerase in the candidate virus for honeybee colony collapse disorder

蜜蜂蜂群崩溃性疾病候选病毒衣壳蛋白和 RNA 聚合酶的结构和功能分析

• 批准号: 23780051
• 负责人: HATAKEYAMA Dai
• 金额: $ 1.75万
• 依托单位: Tokushima Bunri University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780051/
• 关键词: 昆虫病理; IAPV; 殻タンパク質; RNA合成酵素; ミツバチ; 組換えタンパク質

Colony Collapse Disorder (CCD) is a serious problem threatening the health of honeybees and the economic stability of commercial beekeeping and pollination operations in the world. In this study, we focused on Israeli Acute Paralysis Virus (IAPV), which is considered as a candidate reason for CCD, and challenged to investigate the structure and function of the capsid protein and the RNA polymerase in IAPV. Recombinant proteins of the capsid proteins were expressed, solubilized and purified by combinational expression of 2 or 3 kinds of subunit proteins. Next, we found 2 proteins, which seemed to interact with these recombinant capsid protein subunits, from honeybee brains and guts. Two of 6 partial recombinant proteins of RNA polymerase was successful for expression and solubilization. We will try to isolate the proteins in honeybee tissues which interact with these recombinant proteins.

蜂群崩溃病是世界范围内严重威胁蜜蜂健康和商业养蜂授粉经济稳定的重大问题。本研究以以色列急性麻痹病毒(IAPV)为研究对象，对其衣壳蛋白和RNA聚合酶的结构和功能进行了研究。重组衣壳蛋白通过2种或3种亚基蛋白的组合表达、溶解和纯化。接下来，我们从蜜蜂的大脑和肠道中发现了两种似乎与这些重组衣壳蛋白亚基相互作用的蛋白质。RNA聚合酶的6个部分重组蛋白中有2个成功表达和溶解。我们将尝试分离蜜蜂组织中与这些重组蛋白相互作用的蛋白质。

项目成果

インフルエンザウイルスRNA合成酵素における自己アセチル化能の発見

流感病毒RNA合成酶自乙酰化能力的发现

• 发表时间: 2013
• 作者: Kita S;Hashiba R;Ueki S;Kimoto Y;Abe Y;Gotoda Y;Suzuki R;Uraki E;Nara N;Kanazawa A;Hatakeyama D;Kawai R;Fujito Y;Lukowiak K;Ito E.;畠山大,廣田丈典,柳澤伸,長江萌菜美,庄司正樹,葛原隆
• 通讯作者: 畠山大,廣田丈典,柳澤伸,長江萌菜美,庄司正樹,葛原隆

Does Conditioned Taste Aversion Learning in the Pond Snail Lymnaea stagnalis Produce Conditioned Fear?

• DOI: 10.1086/bblv220n1p71
• 发表时间: 2011-02-01
• 期刊: BIOLOGICAL BULLETIN
• 影响因子: 1.6
• 作者: Kita, Serina;Hashiba, Ryuji;Ito, Etsuro
• 通讯作者: Ito, Etsuro

Anti-influenza activity of c60 fullerene derivatives.

• DOI: 10.1371/journal.pone.0066337
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Shoji M;Takahashi E;Hatakeyama D;Iwai Y;Morita Y;Shirayama R;Echigo N;Kido H;Nakamura S;Mashino T;Okutani T;Kuzuhara T
• 通讯作者: Kuzuhara T

Identification and purification of resorcinol, an antioxidant specific to Awa-ban (pickled and anaerobically fermented) tea.

间苯二酚的鉴定和纯化，间苯二酚是阿波班（腌制和厌氧发酵）茶特有的抗氧化剂。

• DOI: 10.1016/j.foodres.2013.05.036
• 发表时间: 2013
• 期刊: Food Research International
• 影响因子: 8.1
• 作者: Hiasa M;Kurokawa M;Ohta K;Esumi T;Akita H;Niki K;Yagi Y;Echigo N;Hatakeyama D;Kuzuhara T.
• 通讯作者: Kuzuhara T.

徳島文理大学薬学部生化学教室

德岛文理大学药学部生物化学系