Characterization of the molecular mechanism of anhydrobiosis and functional gene analysis in the chironomid Polypedilum vanderplanki.

摇蚊Polypedilum vanderplanki 脱水分子机制的表征和功能基因分析。

• 批准号: 23780055
• 负责人: CORNETTE Richard
• 金额: $ 2.75万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780055/
• 关键词: ネムリユスリカ; 乾燥耐性; 酸化ストレス; マイクロアレイ解析; RNAi; 培養細胞; Cnc-C; 細胞周期; 細胞分裂; Keap1

Larvae of the sleeping chironomid (Polypedilum vanderplanki) can survive total desiccation in an ametabolic state known as anhydrobiosis. Gene expression during the recovery process was investigated by microarray. Whereas stress genes, such as antioxidants, were still expressed during the resuming of metabolism just after rehydration, 24 h later, we observed genes involved in the repair of biological molecules. DNA repair is tightly associated with cell cycle but synchronization of cell cycle was not detected during anhydrobiosis. On the other hand, oxidative stress induced anhydrobiosis-related gene expression. Consequently, reactive oxygen species could be the main trigger of anhydrobiosis. Thus, we characterized the transcription factor involved in oxidative stress sensing, Cnc-C, whose expression was up-regulated upon desiccation. RNAi experiments on P. vanderplanki cell cultures suggested that Cnc-C was involved in the induction of anhydrobiosis-related gene expression.

睡眠摇蚊的幼虫（Polypedilum vanderplanki）可以在一种称为脱水的无代谢状态下生存。利用基因芯片技术研究了恢复过程中的基因表达。而应激基因，如抗氧化剂，仍然在恢复代谢过程中表达后，24小时后，我们观察到的基因参与修复的生物分子。DNA修复与细胞周期密切相关，但在脱水过程中没有检测到细胞周期的同步化。另一方面，氧化应激诱导脱水相关基因的表达。因此，活性氧可能是厌氧分解的主要触发因素。因此，我们表征了参与氧化应激传感的转录因子Cnc-C，其表达在干燥后上调。对范氏毕赤酵母细胞培养物的RNAi实验表明，Cnc-C参与诱导脱水相关基因的表达。

项目成果

ネムリユスリカのアンヒドロビオシス誘導メカニズム：酸化ストレスの役割.

摇蚊脱水诱导机制：氧化应激的作用。

• 发表时间: 2012
• 作者: Cornette R;Gusev O;岩田健一;黄川田隆洋;奥田隆
• 通讯作者: 奥田隆

Elucidation of mechanisms underlying desiccation tolerance with exhaustive gene analysis

通过详尽的基因分析阐明干燥耐受性的机制

• 发表时间: 2012
• 期刊: Cryobiol Cryotech
• 作者: Mukae K;Cornette R;Gusev O;Hatanaka R;Okuda T;Kikawada T
• 通讯作者: Kikawada T

ネムリユスリカの乾燥耐性における酸化ストレスの影響

氧化应激对摇摇蚊干燥耐受性的影响

• 作者: Cornette R;岩田健一;金森保志;Gusev O;黄川田隆洋;奥田隆
• 通讯作者: 奥田隆

ユスリカ科における小さいゲノムサイズと極限環境に対する適応能力.

摇蚊科基因组较小，对极端环境的适应性强。

• 发表时间: 2013
• 作者: Cornette R;Gusev O;志村幸子;黄川田隆洋;奥田隆
• 通讯作者: 奥田隆

Oxidative stress and anhydrobiosis in the desiccation-tolerant midge, Polypedilum vanderplanki.

耐干燥蠓的氧化应激和脱水生物，Polypedilum vanderplanki。

• 发表时间: 2014
• 作者: Cornette R;Gusev O;Hatanaka R;Okada J;Kikawada;T;Okuda;T
• 通讯作者: T