Molecular mechanism of phosphate transport in arbuscular mycorrhiza

通过对日本莲LjPAP3基因敲低表型的研究，阐明了磷在丛枝菌根中转运的分子机制。敲除植株在吸收磷酸盐方面存在缺陷。在击倒植株中定植的丛枝菌根真菌比在野生型植株中定植的多磷酸盐要多得多。这些结果表明LjPAP3通过菌根共生参与了磷酸盐的运输。鉴定出丛枝菌根真菌的多磷酸聚合酶：VTC1、VTC2和VTC4。VTC4从ATP生成多磷酸。

Phenotype of LjPAP3 knockdown in Lotus japonicus was evaluated to elucidate the molecular mechanism of phosphate transport in arbuscular mycorrhiza. The knockdown plants were defective in phosphate uptake. Arbuscular mycorrhizal fungi colonized in the knockdown plants had much more polyphosphate than in the wild-type plants. These results suggested that LjPAP3 is involved in phosphate transport via mycorrhizal symbiosis. Polyphosphate polymerases of arbuscular mycorrhizal fungus were identified: VTC1, VTC2 and VTC4. The VTC4 generated polyphosphate from ATP.