Symbiotic phosphate acquisition in mycorrhizal legume

本文采用免疫细胞化学和分子生物学方法对丛枝菌根的磷酸盐转运机制进行了研究。多聚磷酸盐在根内菌丝的液泡和细胞壁中检测到，而在丛枝中几乎检测不到。在丛枝周围检测到酸性磷酸酶活性，说明丛枝细胞壁中的多磷酸盐可能被酸性磷酸酶水解。我们还在百脉根中鉴定了一个紫色酸性磷酸酶基因LjPAP 3，该基因在包括丛枝在内的皮层细胞中特异表达。

We analyzed a phosphate translocation mechanism of arbuscular mycorrhiza by using methods of immunocytochemistry and molecular biology. Polyphosphates were detected in vacuoles and cell walls of intraradical hyphae, but were hardly detected in arbuscules. As an acid phosphatase activity was detected in peri-arbuscular spaces, the polyphosphate in cell wall of arbuscules may be hydrolyzed by the acid phosphatase. We also identified a purple acid phosphatase gene LjPAP3 in Lotus japonicus, which specifically expressed in cortical cells including arbuscules.