Development of genome-engineering technology and its application to genome evolution in Saccharomyces cerevisiae

利用我们开发的聚合酶链式反应介导的染色体复制和缺失技术，我们研究了特定染色体区域的节段性复制或缺失是否改善了酿酒酵母中重要的工业表型。对染色体节段性复制和缺失的系统分析表明，特定染色体区域拷贝数的改变增强了对包括乙醇、高温和酸胁迫在内的各种逆境的抗性。这些结果表明，基因组工程技术不仅为分析基因组功能提供了独特而有用的工具，也为高效生产生物乙醇或有机酸的酿酒酵母育种提供了一个独特而有用的工具。

Using PCR-mediated chromosomal duplication and deletion technologies that we developed, we investigated whether segmental duplication or deletion of specific chromosomal regions improved industrially important phenotypes in Saccharomyces cerevisiae. Systematic analysis of segmental duplication and deletion of chromosomes revealed that alteration of copy number of specific chromosomal regions enhanced resistance against a variety of stresses including ethanol, high-temperature and acid stresses. These results indicate that the genome-engineering technologies provide an unique and useful tool not only for analyzing genome function but also for breeding of S. cerevisaie for efficient production of bioethanol or organic acids.