Molecular mechanisms conferring lactic acid resistance in Saccharomyces cerevisiae

本研究分析了酿酒酵母对乳酸的抗性机制。我们的分析显示，Pbs2激酶和Swi4、Rlm1和Skn7转录激活因子在ESBP6过表达的乳酸抗性中发挥重要作用，ESBP6编码一种功能未知的膜蛋白。乳酸胁迫导致细胞内氨基酸浓度显著降低。ESBP6的过表达减轻了这种减少，特别是参与应激保护的氨基酸，这表明细胞内氨基酸稳态对赋予乳酸抗性很重要。

In this study, resistance mechanisms to lactic acid were analyzed in the yeast Saccharomyces cerevisiae. Our analysis revealed that the Pbs2 kinase and Swi4, Rlm1, and Skn7 transcriptional activators played important roles in the lactic acid resistance conferred by overexpressed ESBP6 that encodes a membrane protein of unknown function. Lactic acid stress caused a significant decrease in intracellular concentration of amino acids. Overexpression of ESBP6 alleviated the decrease, especially amino acids involved in stress protection, suggesting that intracellular amino acid homeostasis is important for conferring lactic acid resistance.