Heterologous secondary metabolite production in genome-minimized Streptomyces host

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We have already demonstrated the heterologous expression of biosynthetic gene clusters in the large-deletion mutants of S. avermitilis (SUKA) that lack major endogenous biosynthetic gene clusters and did not produce these endogenousmetabolites. SUKA have been shown to be versatile and effective hosts for the expression of heterologous gene clusters governing the production of a variety of secondary metabolites. In this study we developed novel marker-less multi gene introduction system using 5 integrating vectors derived from 5 different actinophages. It is useful gene introduction system for production of unnatural compounds by combinatorial biosynthesis.