Development of a novel simultaneous detection and identification method for genetically modified plants using DNA ID tag

转基因作物的数量一直在不断增加，要全面检测所有这些作物几乎是不可能的。作为前期研究，我们培育了导入了多个DNA ID标签的转基因植株，并获得了多个水稻转基因品系。我们利用提取的水稻基因组DNA对该系统进行了评价，并设计了通用引物和特异性探针。采用普通引物对对所引入的DNA标签进行常规PCR检测，采用在标签区域设计的特异性DNA探针对所引入的三个DNA标签进行实时PCR检测。

The number of GM crops has been continuously increasing, and it is virtually impossible to detect comprehensively all of these crops. As a pilot study, we generated GM plants which was introduced several DNA ID tags, and several rice transgenic lines were obtained. We evaluated the system using extracted rice gnomic DNA and designed common primers and specific probes. It was confirmed that the introduced ID tags were detectable with conventional PCR analysis using the common primer pair and the three DNA ID tags were identified with real-time PCR analysis using specific DNA probes which were designed in the ID tag regions.