Development of a novel simultaneous detection and identification method for genetically modified plants using DNA ID tag

转基因作物的数量不断增加，几乎不可能全面检测所有这些作物。作为初步研究，我们在转基因植株中导入了多个DNA ID标签，并获得了多个转基因株系。利用提取的水稻基因组DNA，设计通用引物和特异性探针，对该体系进行了评价。证实了引入的ID标签可通过使用共同引物对的常规PCR分析检测，并且三种DNA ID标签通过使用在ID标签区域中设计的特异性DNA探针的实时PCR分析鉴定。

The number of GM crops has been continuously increasing, and it is virtually impossible to detect comprehensively all of these crops. As a pilot study, we generated GM plants which was introduced several DNA ID tags, and several rice transgenic lines were obtained. We evaluated the system using extracted rice gnomic DNA and designed common primers and specific probes. It was confirmed that the introduced ID tags were detectable with conventional PCR analysis using the common primer pair and the three DNA ID tags were identified with real-time PCR analysis using specific DNA probes which were designed in the ID tag regions.