Search of novel pregnancy recognition substance: Prospects for improvement of conception rate

新型妊娠识别物质的探索：提高受孕率的前景

• 批准号: 23780279
• 负责人: SAKURAI Toshihiro
• 金额: $ 2.91万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780279/
• 关键词: 機能性物質; 着床; 妊娠認識; 細胞不死化; 受胎率向上; PBMCs; インターフェロンタウ; 共培養

The purpose of this study is to appear the maternal changes in the blood or uterine that occur in early pregnancy within 10 days after the artificial insemination (AI), to assist the pregnancy recognition at embryo transfer (ET) to improve the conception rate. However, prior to secrete interferon tau from trophectderm, because it was found that changes in gene expression in the uterus of the mother is not observed , it had to be changed the original plan. We, therefore, aimed to explore new pregnancy recognition factors during peri-attachment period. In this study, results were obtained as following: An in vitro coculture system which mimics bovine attachment process was developed. In addition, immortalized cell of uterine epithelial cells was established. Besides, in vitro coculture system appeared EGF secreted into uterine lumen at the initial of attachment process is important. Thus, this in vitro implantation assessment system could be a useful method not only can determine the factors which are important for attachment, but also to explore the improving drugs for pregnancy rate.

本研究的目的是在人工授精（AI）后10天内出现妊娠早期母体血液或子宫的变化，以辅助胚胎移植（ET）时的妊娠识别，提高受孕率。然而，在从滋养层分泌干扰素tau之前，由于发现母体子宫内基因表达变化未被观察到，不得不改变原计划。因此，我们的目的是探索新的怀孕识别因素，在围依附期。在本研究中，获得了以下结果：建立了一种模拟牛附着过程的体外共培养体系。此外，还建立了子宫上皮细胞的永生化细胞。此外，体外共培养系统中出现的EGF在附着过程初期分泌到子宫腔中也很重要。因此，该体外植入评估系统不仅可以确定附着的重要因素，而且可以探索提高妊娠率的药物。

项目成果

Dynamic evolution of endogenous retrovirus-derived genes expressed in bovine conceptuses during the period of placentation.

• DOI: 10.1093/gbe/evt007
• 发表时间: 2013
• 期刊: Genome biology and evolution
• 影响因子: 3.3
• 作者: Nakagawa S;Bai H;Sakurai T;Nakaya Y;Konno T;Miyazawa T;Gojobori T;Imakawa K
• 通讯作者: Imakawa K

Down-regulation of interferon tau gene transcription with a transcription factor, EOMES

转录因子 EOMES 下调干扰素 tau 基因转录

• DOI: 10.1002/mrd.22171
• 发表时间: 2013
• 期刊: Molecular Reproduction and Development
• 影响因子: 2.5
• 作者: Sakurai T;Bai H;Bai R;Sato D;Arai M;Okuda K;Ideta A;Aoyagi Y;Godkin JD;Imakawa K.
• 通讯作者: Imakawa K.

Transcriptional down-regulation of IFNT expression during the peri-implantation period in ruminants

反刍动物植入期间 IFNT 表达的转录下调

• 发表时间: 2011
• 作者: Sakurai T;Bai H;Konno T;Okuda K;Godkin JD and Imakawa K
• 通讯作者: Godkin JD and Imakawa K

Regulation of Trophoblast-Specific Factors by GATA2 and GATA3 in Bovine Trophoblast CT-1 Cells

• DOI: 10.1262/jrd.10-186k
• 发表时间: 2011-08-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Bai, Hanako;Sakurai, Toshihiro;Imakawa, Kazuhiko
• 通讯作者: Imakawa, Kazuhiko

Establishment of invitro assessment system for conceptus attachment to the uterine epithelial cells

孕体与子宫上皮细胞附着的体外评估体系的建立

• 发表时间: 2011
• 作者: 櫻井敏博;唄花子;荒井未来;奥田潔;今川和彦;櫻井敏博;Sakurai T
• 通讯作者: Sakurai T