Involvement of MARCKS on methylmercury neurotoxicity and application as a marker for neurotoxicity

由于肉豆蔻酰化的富含丙氨酸的C-激酶底物(MARCKS)在神经细胞的分化和发育中起重要作用，我们研究了甲基汞对神经母细胞瘤SH-SY5Y细胞和大鼠脑内MARCKS表达和磷酸化的影响。甲基汞可导致SH-SY5Y细胞存活率下降，并伴有Marcks的磷酸化和表达改变。在甲基汞处理的大鼠脑组织中，观察到异常的MARCKS磷酸化。本研究可能表明MARCKS的改变对甲基汞所致的神经毒性有一定的影响。

Because myristoylated alanine-rich C kinase substrate (MARCKS) plays an essential role in the differentiation and development of neuronal cells, we studied the alteration of MARCKS expression and phosphorylation in MeHg-induced neurotoxicity of neuroblastoma SH-SY5Y cells and in the rat brain. Exposure to MeHg induced a decrease in cell viability of SH-SY5Y cells, which was accompanied by an alteration of MARCKS phosphorylation and expression. In brain tissue from MeHg-treated rats, abnormal MARCKS phosphorylation was observed. The present study may indicate that alteration in MARCKS has consequences for MeHg-induced neurotoxicity.