Involvement of MARCKS on methylmercury neurotoxicity and application as a marker for neurotoxicity

MARCKS对甲基汞神经毒性的参与及其作为神经毒性标志物的应用

• 批准号: 23780298
• 负责人: SHIRAISHI Mitsuya
• 金额: $ 2.75万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780298/
• 关键词: メチル水銀; MARCKS

Because myristoylated alanine-rich C kinase substrate (MARCKS) plays an essential role in the differentiation and development of neuronal cells, we studied the alteration of MARCKS expression and phosphorylation in MeHg-induced neurotoxicity of neuroblastoma SH-SY5Y cells and in the rat brain. Exposure to MeHg induced a decrease in cell viability of SH-SY5Y cells, which was accompanied by an alteration of MARCKS phosphorylation and expression. In brain tissue from MeHg-treated rats, abnormal MARCKS phosphorylation was observed. The present study may indicate that alteration in MARCKS has consequences for MeHg-induced neurotoxicity.

由于富含豆蔻酰丙氨酸的C激酶底物（MARCKS）在神经细胞的分化和发育中起着重要作用，我们研究了甲基汞诱导的神经母细胞瘤SH-SY 5 Y细胞和大鼠脑中MARCKS表达和磷酸化的变化。暴露于甲基汞诱导SH-SY 5 Y细胞的细胞活力下降，这是伴随着MARCKS磷酸化和表达的改变。在甲基汞处理的大鼠脑组织中，观察到异常MARCKS磷酸化。本研究可能表明，MARCKS的改变对甲基汞诱导的神经毒性有影响。

项目成果

Increased expression of aquaporin-4 with methylmercury exposure in the brain of the common marmoset

• DOI: 10.2131/jts.37.749
• 发表时间: 2012-08-01
• 期刊: JOURNAL OF TOXICOLOGICAL SCIENCES
• 影响因子: 2
• 作者: Yamamoto, Megumi;Takeya, Motohiro;Eto, Komyo
• 通讯作者: Eto, Komyo

MPP+誘導性アポトーシスに対するMARCKSの関与．

MARCKS 参与 MPP+ 诱导的细胞凋亡。

• 作者: 下田宙;高橋慧;高崎智彦;近藤高志;下島昌幸;前田健;田辺敦弘，桜井祥太，白石光也，佐々木泰治．
• 通讯作者: 田辺敦弘，桜井祥太，白石光也，佐々木泰治．

Analysis of intracellular calcium mobilization induced by heavy metals using fura-2 and TPEN in SH-SY5Y cells

使用fura-2和TPEN分析SH-SY5Y细胞中重金属诱导的细胞内钙动员

• 发表时间: 2014
• 作者: 大久保正人,宮本篤,白石光也

重金属による細胞内カルシウム濃度の変化とその特徴

重金属引起的细胞内钙浓度变化及其特性

• 发表时间: 2013
• 作者: 野口慧多;下田宙;寺田豊;長尾裕美子;下島昌幸;香山薫;猪島康雄;前田健;大久保正人,宮本篤,白石光也
• 通讯作者: 大久保正人,宮本篤,白石光也

Alteration in MARCKS phosphorylation and expression by methylmercury in SH-SY5Y cells and rat brain.

甲基汞在 SH-SY5Y 细胞和大鼠脑中改变 MARCKS 磷酸化和表达。

• DOI: 10.1016/j.etap.2014.04.025
• 发表时间: 2014
• 期刊: Environmental toxicology and pharmacology
• 影响因子: 4.3
• 作者: M. Shiraishi;Makoto Hangai;Megumi Yamamoto;M. Sasaki;Atsuhiro Tanabe;Y. Sasaki;A. Miyamoto
• 通讯作者: A. Miyamoto