Intravital FRET Monitoring for Systematic Evaluation of Drug Delivery Systems

高效的药物递送系统(DDS)必须满足矛盾的要求，因为它既要保证货物在非靶标环境中的安全，又要保证货物在目标细胞中的释放。结合Forster共振能量转移(FRET)和活体成像技术，评估DDS的活体分布和完整性。从血液循环研究、组织分布、细胞内稳定性和核酸释放等多个方面对DDS的活体性能进行了评估。

Efficient drug delivery system (DDS) must fulfill paradoxical requirements as it should secure the cargo in the off-target environment, and also release the cargo at the target cells. Using a combination of Forster resonance energy transfer (FRET) and intravital imaging techniques, the intravital distribution and intactness of the DDS was evaluated. Intravital performance of the DDS was assessed in many aspects such as blood circulation studies, tissue distribution, intracellular stability, and release of the nucleic acid.