Analysis of the regulatory mechanism and function of stathmin1 in the central nervous system

Stathmin1中枢神经系统调控机制及功能分析

• 批准号: 23790227
• 负责人: YAMADA Kohei
• 金额: $ 2.83万
• 依托单位: Hamamatsu University School of Medicine (2013)Osaka University (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790227/
• 关键词: stathmin1; 神経; リン酸化タンパク質; 脳発達; 周産期; リン酸化; 脳形成; BDNF; 細胞骨格; 神経発生

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide involved in a variety of brain function. We demonstrated that PACAP regulates stathmin1 expression. Shumyatsky GP. et al. reported that mice without stathmin1 have deficiency in innate and learned fear. However, the detailed function and regulatory mechanism of stathmin1 in the central nervous system are unclear. In this study, we found that stathmin1 was phosphorylated during perinatal stage in the cortex. Phosphorylated stathmin1 was localized in the marginal zone of cortex, forming dot like fibers. In primary cultured neuron study, we detected dot-like phosphorylated stathmin1. And we indicated that BDNF was related to phosphorylation of stathmin1. PC12 cells over-expressed stathmin1 constitutive active form had longer thick neurites and numerous slight neurites as a filopodia. These results show that phosphorylated stathmin1 is related to filopodia or synapse constitution during perinatal period.

腺苷酸环化酶激活多肽（PACAP）是一种参与多种脑功能的神经肽。我们证明了PACAP调节stathmin 1的表达。舒米亚茨基站等人报道了没有stathmin 1的小鼠在先天性和习得性恐惧方面存在缺陷。然而，stathmin 1在中枢神经系统中的详细功能和调节机制尚不清楚。在这项研究中，我们发现stathmin 1磷酸化在围产期在皮层。磷酸化stathmin 1定位于皮质边缘区，形成点状纤维。在原代培养的神经元研究中，我们检测到斑点状磷酸化stathmin 1。我们发现BDNF与stathmin 1的磷酸化有关。过表达stathmin 1组成型活性形式的PC12细胞具有较长较粗的神经突和许多纤细的神经突，呈丝状伪足。这些结果表明，磷酸化stathmin 1在围产期与丝状伪足或突触的构成有关。

项目成果

DISC1 (disrupted-in-schizophrenia-1) regulates differentiation of oligodendrocytes.

• DOI: 10.1371/journal.pone.0088506
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Hattori T;Shimizu S;Koyama Y;Emoto H;Matsumoto Y;Kumamoto N;Yamada K;Takamura H;Matsuzaki S;Katayama T;Tohyama M;Ito A
• 通讯作者: Ito A

TRAP1 controls mitochondrial fusion/fission balance through Drp1 and Mff expression.

• DOI: 10.1371/journal.pone.0051912
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Takamura H;Koyama Y;Matsuzaki S;Yamada K;Hattori T;Miyata S;Takemoto K;Tohyama M;Katayama T
• 通讯作者: Katayama T

Increased stathmin1 expression in the dentate gyrus causes abnormal axonal arborizations.

齿状回中 stathmin1 表达增加会导致轴突分枝异常。

• 作者: Yamada Kohei;Matsuzaki Shinsuke;Hattori Tsuyoshi;Shintani Norihito;Tohyama Masaya;Katayama Taiichi;Suzuki Katsuaki and Mori Norio.
• 通讯作者: Suzuki Katsuaki and Mori Norio.

XLMR protein related to neurite extension (Xpn/KIAA2022) regulates cell-cell and cell-matrix adhesion and migration

• DOI: 10.1016/j.neuint.2013.09.011
• 发表时间: 2013-11-01
• 期刊: NEUROCHEMISTRY INTERNATIONAL
• 影响因子: 4.2
• 作者: Magome, Takuya;Hattori, Tsuyoshi;Katayama, Taiichi
• 通讯作者: Katayama, Taiichi

Implications for Human Diseases, Neuronal SUMOylation and Its Contributions to Form, Function and Neurodegenerative Diseases

对人类疾病的影响、神经元 SUMO 化及其对形式、功能和神经退行性疾病的贡献

• 发表时间: 2012
• 作者: Shinsuke Matsuzaki;Kohei Yamada;Nicola St George-Hyslop;Brian Raught;Taiichi Katayama;Masaya Tohyama and Paul Fraser
• 通讯作者: Masaya Tohyama and Paul Fraser