Molecular mechanisms underlying the glia-mediated osmo-regulation

我们的特点是三个秀丽隐杆线虫HMIT基因，hmit-1.1，hmit-1.2，和hmit-1.3。HMIT-1.1在肠中表达，HMIT-1.2在神经胶质和排泄管中表达，排泄管是功能上类似于肾的渗透调节器官。HMIT-1.3在肠和胶质细胞中表达。在高渗条件下，hmit-1.1和hmit-1.2的表达被显著诱导，但hmit-1.3的表达不被显著诱导。突变型hmit-1.1和hmit-1.2的动物对渗透压应激具有高敏感性。hmit-1.1和hmit-1.2突变体的缺陷分别被hmit-1.1和hmit-1.2转基因以及修饰的人HMIT拯救。在人细胞系中，HMIT表达在高渗条件下诱导。这些结果表明，C。HMIT家族在线虫的保护性反应中起着至关重要的作用。

We characterized three Caenorhabditis elegansHMIT genes, hmit-1.1, hmit-1.2, and hmit-1.3. hmit-1.1 was expressed in the intestine, and hmit-1.2 was expressed in the glia and the excretory canal, which is an osmotic regulatory organ that is functionally analogous to the kidney. hmit-1.3 was expressed in the intestine and the glia. The expression of hmit-1.1 and hmit-1.2 but not hmit-1.3, was markedly induced under hyperosmotic conditions. Animals with mutant hmit-1.1 and hmit-1.2 were hypersensitive to osmotic stress. The defects of hmit-1.1 and hmit-1.2 mutants were rescued by hmit-1.1 and hmit-1.2 transgenes, respectively, and by modified human HMIT. In human cell lines, HMIT expression was induced in hyperosmotic conditions. These findings indicate that the C. elegans HMIT family has a crucial role in the osmoprotective response.