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Molecular mechanisms underlying the glia-mediated osmo-regulation

胶质细胞介导的渗透调节的分子机制

基本信息

批准号：
23790256
负责人：
KAGE-NADAKAI Eriko
金额：
$ 2.66万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790256/
关键词：
浸透圧グリア細胞線虫生理学神経科学

项目摘要

We characterized three Caenorhabditis elegansHMIT genes, hmit-1.1, hmit-1.2, and hmit-1.3. hmit-1.1 was expressed in the intestine, and hmit-1.2 was expressed in the glia and the excretory canal, which is an osmotic regulatory organ that is functionally analogous to the kidney. hmit-1.3 was expressed in the intestine and the glia. The expression of hmit-1.1 and hmit-1.2 but not hmit-1.3, was markedly induced under hyperosmotic conditions. Animals with mutant hmit-1.1 and hmit-1.2 were hypersensitive to osmotic stress. The defects of hmit-1.1 and hmit-1.2 mutants were rescued by hmit-1.1 and hmit-1.2 transgenes, respectively, and by modified human HMIT. In human cell lines, HMIT expression was induced in hyperosmotic conditions. These findings indicate that the C. elegans HMIT family has a crucial role in the osmoprotective response.

我们鉴定了三个秀丽隐杆线虫的shmit基因，分别是hmit-1.1、hmit-1.2和hmit-1.3。Hmit-1.1在肠中表达，而hmit-1.2在胶质细胞和排泄管中表达，排泄管是一个功能类似肾脏的渗透调节器官。Hmit-1.3在肠和胶质细胞中表达。高渗条件下显著诱导hmit1 1和hmit2 2表达，但不诱导hmit1 3 3表达。突变hmit1 -1和hmit1 - 2的动物对渗透胁迫过敏。HMIT -1.1和HMIT -1.2突变体的缺陷分别通过HMIT -1.1和HMIT -1.2转基因和修饰的人HMIT修复。在人类细胞系中，HMIT在高渗条件下被诱导表达。这些发现表明秀丽隐杆线虫HMIT家族在渗透保护反应中起着至关重要的作用。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The homologous carboxyl-terminal domains of microtubule-associated protein 2 and TAU induce neuronal dysfunction and have differential fates in the evolution of neurofibrillary tangles.

DOI：
10.1371/journal.pone.0089796
发表时间：
2014
期刊：
PloS one
影响因子：
3.7
作者：
Xie C;Miyasaka T;Yoshimura S;Hatsuta H;Yoshina S;Kage-Nakadai E;Mitani S;Murayama S;Ihara Y
通讯作者：
Ihara Y

NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane.

DOI：
10.1371/journal.pone.0043050
发表时间：
2012
期刊：
PloS one
影响因子：
3.7
作者：
Hagiwara K;Nagamori S;Umemura YM;Ohgaki R;Tanaka H;Murata D;Nakagomi S;Nomura KH;Kage-Nakadai E;Mitani S;Nomura K;Kanai Y
通讯作者：
Kanai Y

Academic press.