Multifaceted function analyses of fever-developing neurons using genetically modified rats

使用转基因大鼠对发热神经元进行多方面功能分析

• 批准号: 23790271
• 负责人: NAKAMURA Yoshiko
• 金额: $ 2.83万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790271/
• 关键词: 温熱生理学; 生体恒常性; プロスタグランジン; 発熱; 視索前野; 遺伝子改変動物; 光遺伝学

To reveal the core brain mechanism of fever development, I have performed multifaceted analyses of prostaglandin EP3 receptor-expressing neurons in the preoptic area (EP3 neurons), a center for fever development. By creating a novel transgenic rat and viruses, I established a system that allows us to specifically transduce EP3 neurons with a reporter gene. Using this system, I transduced EP3 neurons with a membrane-targeted form of GFP to visualize their axon fibers, and thereby, successfully identified all the brain regions where EP3 neurons project their axons. Also, by illuminating EP3 neurons transduced with channelrhodopsin, I was able to change thermogenic activity of brown adipose tissue in anesthetized rats. I am currently doing electrophysiological analyses of EP3 neurons in brain slices.

为了揭示发烧发展的核心大脑机制，我对发烧发展中心视前区(EP3神经元)中前列腺素EP3受体表达的神经元进行了多方面的分析。通过创造一种新的转基因大鼠和病毒，我建立了一个系统，允许我们用报告基因特异性地转导EP3神经元。使用这个系统，我用膜靶向形式的GFP转导EP3神经元以显示它们的轴突纤维，从而成功地识别了EP3神经元投射轴突的所有大脑区域。此外，通过照亮通道视紫红质转导的EP3神经元，I能够改变麻醉大鼠棕色脂肪组织的生热活性。我目前正在对脑片中的EP3神经元进行电生理分析。

项目成果

Social defeat stress-induced hyperthermia is associated with neuronal activation of the dorsomedial hypothalamus.

社交失败压力引起的体温过高与背内侧下丘脑的神经元激活有关。

• 发表时间: 2012
• 作者: Oka;T.;Lkhagvasuren;B.;Nakamura;Y. Hayashi;H. & Nakamura;K.
• 通讯作者: K.

Neuropeptide Y in the paraventricular hypotharamic nucreus inhibits brown adipose tissue thermogenesis through GABA inhibition of the rostral medullary raphe

室旁下丘脑核中的神经肽 Y 通过 GABA 抑制延髓中缝头抑制棕色脂肪组织产热

• 发表时间: 2013
• 作者: Nakamura;Y. & Nakamura;K.
• 通讯作者: K.

Medullary GABA neurons that inhibit sympathetic premotor neurons controlling brown adipose tissue thermogenesis.

髓质 GABA 神经元抑制控制棕色脂肪组织产热的交感前运动神经元。

• 发表时间: 2012
• 作者: Nakamura;Y.;Nakamura;K.;Yanagawa;Y. & Morrison;S.F.
• 通讯作者: S.F.

Preoptic regulation of body temperature and fever.

体温和发烧的视前调节。

• 发表时间: 2012
• 作者: Nakamura;Y. & Nakamura;K.
• 通讯作者: K.

Medullary reticular GABAergic neurons that control brown adipose tissue thermogenesis throughinhibition of sympathetic premotor neurons

通过抑制交感前运动神经元控制棕色脂肪组织产热的髓质网状 GABA 能神经元

• 发表时间: 2011
• 作者: Nakamura;Y.;Nakamura;K.;Yanagawa;Y. & Morrison;S.F.
• 通讯作者: S.F.