Investigation of apoC-III on atherosclerosis

兔的脂蛋白代谢特点不同于小鼠，而与人相似，本研究拟制备人apoCIII转基因（Tg）兔，以揭示其在脂蛋白代谢和动脉粥样硬化形成中的作用。在第一年，我们注射了500多个胚胎，但由于出生的活仔数量很少，未能获得任何阳性Tg兔。在此之后，我们修改了显微注射的方案。在第二年，我们成功地获得了48只活着的幼崽。尽管我们仍然没有得到任何携带人类apoCIII基因的阳性兔子，但我们很有可能很快就会成功。正在进行进一步的实验工作。

Features of lipoprotein metabolism in rabbits, unlike mouse, resemble to human, we planned to make human apoCIII transgenic (Tg) rabbits to reveal its functions on lipoprotein metabolism and atherogenesis. For the first year, we injected more than 500 embryos but failed to obtain any positiveTg rabbits due to low number of living pups born. After that, we modified the protocol of microinjection. In the second year, we successfully got 48 alive pups. Although we still did not get any positive rabbits that carry human apoCIII gene, it is quite possible that we will be successful soon. The work is under way for the further experiment.