Tumor-stromal cell interaction and epithelial-mesenchymal transition by secreted-microRNA

分泌型 microRNA 的肿瘤-基质细胞相互作用和上皮-间质转化

• 批准号: 23790403
• 负责人: SAKAMOTO Naoya
• 金额: $ 2.75万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790403/
• 关键词: 消化器; 唾液腺; microRNA; 分泌型miRNA; 胃がん; 分泌型microRNA; 上皮間葉転換

It has been reported that micro-environment in cancer tissue plays important roles in cancer development in addition to genetic abnormalities in cancer cell. Moreover, it is generally accepted that epithelial-mesenchymal transition of cancer cell contributes to tumor invasion and metastasis. On the other hands, it is revealed that microRNA can regulate hundreds of genes and affect biological characteristics of cancer cell. In recent year, it was confirmed that secreted-microRNA which is released through small vesicle into extra-cellular space is stably present, and many researchers focused on its biological significance. In this present study, we perform comprehensive and systematic microRNA expression analysis in cancer stromal tissue and aim to elucidate the role of secreted-microRNA in tumor-stromal cell interaction and epithelial-mesenchymal transition.

据报道，除了癌细胞的遗传异常之外，癌组织中的微环境在癌症的发展中起重要作用。此外，一般认为癌细胞的上皮间质转化有助于肿瘤的侵袭和转移。另一方面，研究发现microRNA可以调控数百个基因，影响癌细胞的生物学特性。近年来，分泌型microRNA通过小泡囊释放到细胞外空间，并稳定存在于细胞内，其生物学意义引起了众多研究者的关注。在本研究中，我们进行了全面和系统的microRNA表达分析，在癌间质组织，旨在阐明分泌的microRNA在肿瘤间质细胞相互作用和上皮间质转化的作用。

项目成果

胃癌で発現が低下しているmiR-148aはMMP7を標的とし,浸潤に関与する

miR-148a在胃癌中表达降低，靶向MMP7并参与侵袭

• 发表时间: 2012
• 作者: Oue N;Sakamoto N;Naito Y;Sentani K;Harris CC and Yasui W;Yasui W;Yasui W;内藤寛,坂本直也,浦岡直礼,仙谷和弘,大上直秀,安井弥;坂本直也,内藤寛,仙谷和弘,大上直秀,安井弥
• 通讯作者: 坂本直也,内藤寛,仙谷和弘,大上直秀,安井弥

Identification of Transmembrane Protein in Prostate Cancer by the Escherichia coli Ampicillin Secretion Trap: Expression of CDON Is Involved in Tumor Cell Growth and Invasion

• DOI: 10.1159/000329588
• 发表时间: 2011-01-01
• 期刊: PATHOBIOLOGY
• 影响因子: 5
• 作者: Hayashi, Tetsutaro;Oue, Naohide;Yasui, Wataru
• 通讯作者: Yasui, Wataru

Cytokeratin is a Predictive Marker for Survival in Patients with Esophageal Squamous Cell Carcinoma

细胞角蛋白是食管鳞状细胞癌患者生存的预测标志物

• 发表时间: 2012
• 期刊: Annals of Surgical Oncology
• 影响因子: 3.7
• 作者: 北川昌伸;倉田盛人;阿部晋也;鈴木志保;梅田茂明;菅原江美子;大西威一郎;Naohide Oue et al
• 通讯作者: Naohide Oue et al

MicroRNA-155 is a predictive marker for survival in patients with clear cell renal cell carcinoma

• DOI: 10.1111/j.1442-2042.2012.03182.x
• 发表时间: 2013-05-01
• 期刊: INTERNATIONAL JOURNAL OF UROLOGY
• 影响因子: 2.6
• 作者: Shinmei, Shunsuke;Sakamoto, Naoya;Yasui, Wataru
• 通讯作者: Yasui, Wataru

Cytokeratin 7 is a Predictive Marker for Survival in Patients with Esophageal Squamous Cell Carcinoma

• DOI: 10.1245/s10434-011-2175-4
• 发表时间: 2012-06-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Oue, Naohide;Noguchi, Tsuyoshi;Yasui, Wataru
• 通讯作者: Yasui, Wataru