Feature analysis of genomic instability using a levelof 53BP1 nuclear focus in esophageal intraepithelial neoplasm

基因组不稳定性(GIN)通常被认为是任何致癌过程的中心方面，并与恶性潜能相关。我们研究了GIN在食道癌发生中的意义。我们使用定位于DNA双链断裂并形成离散核焦点的53BP1蛋白来检测杜松子酒。在本研究中，随着食管异型增生分级的增加，53BP1的表达增加。这表明食道肿瘤中DNA损伤反应的结构性激活，并随着癌症的进展而增加GIN。免疫荧光法检测53BP1的表达可作为判断人上皮内肿瘤恶性潜能的有用组织学指标。

Genomic instability (GIN) is generally considered to be a central aspect of any carcinogenic process and is correlated with malignant potential. We have examined significance of GIN in esophageal carcinogenesis. We use a protein of 53BP1 localized at the sites of DNA double strand breaks and forms discrete nuclear foci to detect GIN. In this study, grade of esophageal dysplasia increases, expression of 53BP1 was increased. This suggested a constitutive activation of DNA damage response in esophageal tumors and increased GIN with progression of cancer. The detection of 53BP1 expression by immunofluorescence can be a useful histological marker to estimate the malignant potential of human esophageal intraepithelial neoplasm.