Detection of genomic instability using a level of 53BP1 nuclear focus in Atomic bomb survivors tissue

我们研究了原子弹幸存者和原子弹辐射暴露与癌症的关系。我们估计辐射效应的特征（基因组不稳定性：GIN）仍然存在于原子弹幸存者的细胞中，并且GIN诱导致癌。本研究旨在检测原子弹幸存者正常皮肤组织中是否存在GIN。我们利用定位于DNA双链断裂位点的53BP1蛋白形成离散核病灶来检测GIN。本研究结果提示近端组（离震源较近的组）正常细胞中GIN升高，GIN诱发的辐射与原子弹幸存者的癌变有关。

We have examined associations with cancer in atomic bomb (A-bomb) survivor and A-bomb radiation exposure. We have estimated that the signature of radiation effect (genomic instability : GIN) remains in A-bomb survivors' cells and GIN induce carcinogenesis. This study aims to detect the existence of GIN in normal skin tissue in A-bomb surviviors. We use a protein of 53BP1 localized at the sites of DNA double strand breaks and forms discrete nuclear foci to detect GIN. The results of this study suggest GIN in normal cells of the proximal group (shorting distance group from the hypocenter) is increasing, and GIN induced radiation is associated with A-bomb survivors' carcinogenesis.